5,7-dihydroxy-3,4,6-trimethoxyflavone inhibits the inflammatory effects induced by Bacteroides fragilis enterotoxin via dissociating the complex of heat shock protein 90 and I kappa B alpha and I kappa B kinase-gamma in intestinal epithelial cell culture
DC Field | Value | Language |
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dc.contributor.author | Kim, J. M. | - |
dc.contributor.author | Lee, D. H. | - |
dc.contributor.author | Kim, J. S. | - |
dc.contributor.author | Lee, J. Y. | - |
dc.contributor.author | Park, H. -G. | - |
dc.contributor.author | Kim, Y. -J. | - |
dc.contributor.author | Oh, Y. -K. | - |
dc.contributor.author | Jung, H. C. | - |
dc.contributor.author | Kim, S. I. | - |
dc.date.accessioned | 2021-09-08T19:27:41Z | - |
dc.date.available | 2021-09-08T19:27:41Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-03 | - |
dc.identifier.issn | 0009-9104 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120521 | - |
dc.description.abstract | Enterotoxin produced by enterotoxigenic Bacteroides fragilis (BFT) has been associated with mucosal inflammation and diarrhoeal diseases. In this study, the anti-inflammatory molecular mechanism of 5,7-dihydroxy-3,4,6-trimethoxyflavone (eupatilin) was characterized in an HT-29 intestinal epithelial cell line stimulated with BFT. Pre-treatment of HT-29 cells with eupatilin decreased the production significantly of both interleukin (IL)-8 and prostaglandin E-2 induced by BFT in a dose-dependent manner. BFT-activated nuclear factor-kappaB (NF-kappa B) signals in HT-29 cells and pretreatment with eupatilin suppressed NF-kappa B activation that resulted in the significant inhibition of IL-8 and cyclo-oxygenase-2 expression. BFT-induced phosphorylation of both I kappa B alpha and I kappa B kinase (IKK) signals was prevented in eupatilin-pretreated HT-29 cells. Transfection of siRNA for IKK-alpha and IKK-beta decreased the production of IL-8 and prostaglandin E-2; however, the transfection of IKK-beta siRNA showed a more significant reduction of BFT-induced I kappa B alpha phosphorylation compared with that of IKK-alpha siRNA. In addition, herbimycin A, a specific inhibitor of heat shock protein 90 (Hsp90), decreased the BFT-induced activation of IKK and NF-kappa B, suggesting that Hsp90 is associated with a pathway of IKK-NF-kappa B-IL-8/cyclo-oxygenase-2 gene signalling. Furthermore, eupatilin dissociated the complex between Hsp90 and IKK-gamma in BFT-stimulated HT-29 cells. These results suggest that eupatilin can suppress the NF-kappa B signalling pathway by targeting the Hsp90-IKK-gamma complex in intestinal epithelial cells and may attenuate BFT-induced inflammatory responses. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | HSP90 COMPLEX | - |
dc.subject | IKK-GAMMA | - |
dc.subject | ACTIVATOR PROTEIN-1 | - |
dc.subject | ARTEMISIA PLANTS | - |
dc.subject | EXPRESSION | - |
dc.subject | CYCLOOXYGENASE-2 | - |
dc.subject | SECRETION | - |
dc.subject | EUPATILIN | - |
dc.subject | APOPTOSIS | - |
dc.subject | PHOSPHORYLATION | - |
dc.title | 5,7-dihydroxy-3,4,6-trimethoxyflavone inhibits the inflammatory effects induced by Bacteroides fragilis enterotoxin via dissociating the complex of heat shock protein 90 and I kappa B alpha and I kappa B kinase-gamma in intestinal epithelial cell culture | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Y. -K. | - |
dc.identifier.doi | 10.1111/j.1365-2249.2008.03849.x | - |
dc.identifier.scopusid | 2-s2.0-59249100492 | - |
dc.identifier.wosid | 000262950100020 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL IMMUNOLOGY, v.155, no.3, pp.541 - 551 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL IMMUNOLOGY | - |
dc.citation.title | CLINICAL AND EXPERIMENTAL IMMUNOLOGY | - |
dc.citation.volume | 155 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 541 | - |
dc.citation.endPage | 551 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | HSP90 COMPLEX | - |
dc.subject.keywordPlus | IKK-GAMMA | - |
dc.subject.keywordPlus | ACTIVATOR PROTEIN-1 | - |
dc.subject.keywordPlus | ARTEMISIA PLANTS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | EUPATILIN | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordAuthor | Bacteroides fragilis enterotoxin | - |
dc.subject.keywordAuthor | eupatilin | - |
dc.subject.keywordAuthor | Hsp90 | - |
dc.subject.keywordAuthor | I kappa B kinase | - |
dc.subject.keywordAuthor | NF-kappa B | - |
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