Targeted delivery of siRNA against hepatitis C virus by apolipoprotein A-I-bound cationic liposomes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Soo In | - |
dc.contributor.author | Shin, Duckhyang | - |
dc.contributor.author | Lee, Hyeon | - |
dc.contributor.author | Ahn, Byung-Yoon | - |
dc.contributor.author | Yoon, Yeup | - |
dc.contributor.author | Kim, Meehyein | - |
dc.date.accessioned | 2021-09-08T19:30:55Z | - |
dc.date.available | 2021-09-08T19:30:55Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-03 | - |
dc.identifier.issn | 0168-8278 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120538 | - |
dc.description.abstract | Background/Aims: Hepatitis C virus (HCV) is one of the major human hepatic RNA viruses. Recently, we developed a liver-specific siRNA delivery technology using DTC-Apo composed of cationic liposomes (DTC) and apolipoprotein A-I (apo A-I). Here, we investigated whether DTC-Apo nanoparticles can systemically deliver siRNA into mouse hepatocytes expressing HCV proteins and inhibit their expression efficiently. Methods: A transient HCV model was constructed by hydrodynamic injection of plasmid DNA expressing viral structural proteins under hepatic control region and alpha 1-antitrypsin promoter elements. Using this model, DTC-Apo containing HCV-core-specific siRNA was intravenously injected to assess antiviral activity as well as the duration of silencing. Results: Post-administration of DTC-Apo/HCV-specific siRNA at a dose of 2 mg siRNA/kg inhibited viral gene expression by 65-75% in the liver on day 2. Improved activity (95% knockdown on day 2) without immunotoxicity was obtained by 2'-OMe-modification at two U sequences on its sense strand. Notably, the gene silencing effect of the modified siRNA was still maintained at day 6, while the unmodified one lost RNAi activity after day 4. Conclusions: Our results suggest that DTC-Apo liposome is a highly potential delivery vehicle to transfer therapeutic siRNA especially targeting HCV to the liver. (C) 2008 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | SMALL INTERFERING RNAS | - |
dc.subject | B TYPE-I | - |
dc.subject | SCAVENGER RECEPTOR | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | LIVER | - |
dc.subject | MICE | - |
dc.subject | VIVO | - |
dc.subject | INHIBITION | - |
dc.subject | LOCALIZATION | - |
dc.subject | REPLICATION | - |
dc.title | Targeted delivery of siRNA against hepatitis C virus by apolipoprotein A-I-bound cationic liposomes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Byung-Yoon | - |
dc.identifier.doi | 10.1016/j.jhep.2008.10.029 | - |
dc.identifier.scopusid | 2-s2.0-59349118475 | - |
dc.identifier.wosid | 000264224300007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF HEPATOLOGY, v.50, no.3, pp.479 - 488 | - |
dc.relation.isPartOf | JOURNAL OF HEPATOLOGY | - |
dc.citation.title | JOURNAL OF HEPATOLOGY | - |
dc.citation.volume | 50 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 479 | - |
dc.citation.endPage | 488 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | SMALL INTERFERING RNAS | - |
dc.subject.keywordPlus | B TYPE-I | - |
dc.subject.keywordPlus | SCAVENGER RECEPTOR | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Apolipoprotein A-I | - |
dc.subject.keywordAuthor | Hepatitis C virus | - |
dc.subject.keywordAuthor | Systemic delivery | - |
dc.subject.keywordAuthor | Tissue microarray | - |
dc.subject.keywordAuthor | SR-BI | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.