Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nam, Ki Hyun | - |
dc.contributor.author | Kim, Min-Young | - |
dc.contributor.author | Kim, Soo-Jin | - |
dc.contributor.author | Priyadarshi, Amit | - |
dc.contributor.author | Lee, Won Ho | - |
dc.contributor.author | Hwang, Kwang Yeon | - |
dc.date.accessioned | 2021-09-08T19:54:41Z | - |
dc.date.available | 2021-09-08T19:54:41Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009-02-06 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120587 | - |
dc.description.abstract | Hormone-sensitive lipase (HSL) plays an important role in the regulation of rodent fat cell lipolysis. It is regarded as an adipose tissue-specific enzyme whose sole metabolic role is the catalysis of hormone-stimulated lipolysis in mammalian cells. In this report we describe the functional and structural analysis of an EstE5 protein from a soil metagenome library. Function analysis results indicated that EstE5 preferentially hydrolyzes short-chain ester compounds, and our kinetic Studies revealed the optimal pH and temperature. Based on the structural analysis, we defined the active site and the binding pocket. Structurally, EstE5 belongs to the HSL family and these structural studies may have applications in the production Of Value-added products, including pharmaceuticals. (C) 2008 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | THERMOPHILIC ESTERASE | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | FOLD FAMILY | - |
dc.subject | GENE | - |
dc.subject | SYSTEM | - |
dc.title | Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Kwang Yeon | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.12.085 | - |
dc.identifier.scopusid | 2-s2.0-58149525330 | - |
dc.identifier.wosid | 000262924300074 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.379, no.2, pp.553 - 556 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 379 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 553 | - |
dc.citation.endPage | 556 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | THERMOPHILIC ESTERASE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | FOLD FAMILY | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | EstE5 | - |
dc.subject.keywordAuthor | Esterase/lipase | - |
dc.subject.keywordAuthor | Hormone sensitive lipase | - |
dc.subject.keywordAuthor | HSL family | - |
dc.subject.keywordAuthor | Soil metagenome library | - |
dc.subject.keywordAuthor | Crystal structure | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.