The Effect of Saccharomyces boulardii on Human Colon Cells and Inflammation in Rats with Trinitrobenzene Sulfonic Acid-Induced Colitis
DC Field | Value | Language |
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dc.contributor.author | Lee, Sang Kil | - |
dc.contributor.author | Kim, Youn Wha | - |
dc.contributor.author | Chi, Sung-Gil | - |
dc.contributor.author | Joo, Yeong-Shil | - |
dc.contributor.author | Kim, Hyo Jong | - |
dc.date.accessioned | 2021-09-08T20:12:02Z | - |
dc.date.available | 2021-09-08T20:12:02Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009-02 | - |
dc.identifier.issn | 0163-2116 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120657 | - |
dc.description.abstract | Saccharomyces boulardii (S. boulardii) has beneficial effects in the treatment of intestinal inflammation; however, little is known about the mechanisms by which these effects occur. We investigated the effects of S. boulardii on the expression of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and interleukin-8 (IL-8), using human HT-29 colonocytes and a rat model of trinitrobenzene sulfonic acid (TNBS)-induced colitis. The effect of S. boulardii on gene expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and Northern blot and Western blot assays. Pharmacological inhibitors for various signaling pathways were used to determine the signaling pathways implicated in the S. boulardii regulation of PPAR-gamma and IL-8. We found that S. boulardii up-regulated and downregulated PPAR-gamma and IL-8 expression at the transcription level, both in vitro and in vivo (P < 0.05, respectively). Saccharomyces boulardii blocked tumor necrosis factoralpha (TNF-alpha) regulation of PPAR-gamma and IL-8 through disruption of TNF-alpha-mediated nuclear factor kappa B (NF-kappa B) activation. Furthermore, S. boulardii suppressed colitis and expression of pro-inflammatory cytokine genes in vivo (P < 0.05, respectively). Our study demonstrated that S. boulardii reduces colonic inflammation and regulates inflammatory gene expression. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | PROLIFERATOR-ACTIVATED RECEPTOR | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | BOWEL-DISEASE | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | PPAR-GAMMA | - |
dc.subject | CROHNS-DISEASE | - |
dc.subject | MODELS | - |
dc.subject | ALPHA | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | UBIQUITINATION | - |
dc.title | The Effect of Saccharomyces boulardii on Human Colon Cells and Inflammation in Rats with Trinitrobenzene Sulfonic Acid-Induced Colitis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chi, Sung-Gil | - |
dc.identifier.doi | 10.1007/s10620-008-0357-0 | - |
dc.identifier.scopusid | 2-s2.0-59449084229 | - |
dc.identifier.wosid | 000262968200011 | - |
dc.identifier.bibliographicCitation | DIGESTIVE DISEASES AND SCIENCES, v.54, no.2, pp.255 - 263 | - |
dc.relation.isPartOf | DIGESTIVE DISEASES AND SCIENCES | - |
dc.citation.title | DIGESTIVE DISEASES AND SCIENCES | - |
dc.citation.volume | 54 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 255 | - |
dc.citation.endPage | 263 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | PROLIFERATOR-ACTIVATED RECEPTOR | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | BOWEL-DISEASE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | CROHNS-DISEASE | - |
dc.subject.keywordPlus | MODELS | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | UBIQUITINATION | - |
dc.subject.keywordAuthor | Saccharomyces boulardii | - |
dc.subject.keywordAuthor | PPAR-gamma | - |
dc.subject.keywordAuthor | IL-8 | - |
dc.subject.keywordAuthor | HT-29 | - |
dc.subject.keywordAuthor | TNBS-induced colitis | - |
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