Opsonized erythrocyte ghosts for liver-targeted delivery of antisense oligodeoxynucleotides
DC Field | Value | Language |
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dc.contributor.author | Kim, Sang-Hee | - |
dc.contributor.author | Kim, Eun-Joong | - |
dc.contributor.author | Hou, Joon-Hyuk | - |
dc.contributor.author | Kim, Jung-Mogg | - |
dc.contributor.author | Choi, Han-Gon | - |
dc.contributor.author | Shim, Chang-Koo | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2021-09-08T20:15:51Z | - |
dc.date.available | 2021-09-08T20:15:51Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009-02 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120676 | - |
dc.description.abstract | The use of antisense oligodeoxynucleotides (AS-ODNs) in therapeutic applications requires the development of appropriate analysis and delivery systems. Here, we report a quantitation method and a carrier-mediated AS-ODN delivery system. AS-ODN levels were quantitated using an enzyme-linked immunosorbent assay (ELISA) in which biotinylated AS-ODNs bound to streptavidin-coated plates were detected by binding of a complementary, dinitrophenol-labeled detector ODN. The ELISA-based assay could detect AS-ODNs at the femtomole level. AS-ODN delivery systems based on opsinized erythrocyte ghosts (EGs) were developed using various combinations of hypotonic solution and resealing buffer to optimize AS-ODN encapsulation efficiencies. AS-ODN and polyethylene imine (PEI) complex formation did not affect encapsulation into EGs. The ELISA-based assay showed that the pharmacokinetics of AS-ODNs differed significantly among the various delivery methods. Opsonized EG-encapsulated AS-ODNs exhibited a mean residence time (MRT) significantly shorter than AS-ODN encapsulated in EGs. The biodistribution of EG-loaded AS-ODNs depended on opsonization, with opsonized EG carriers producing 4.5-fold higher levels of AS-ODN in the liver compared with unopsonized EGs. These results indicate that opsonized EGs can be used for liver-targeted delivery of AS-ODN and suggest that an ELISA-based method may be useful for studying the in vivo fate of AS-ODNs. (C) 2008 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | OLIGONUCLEOTIDES | - |
dc.subject | PLASMA | - |
dc.subject | PHOSPHOROTHIOATE | - |
dc.subject | PHAGOCYTOSIS | - |
dc.subject | THERAPY | - |
dc.subject | SYSTEM | - |
dc.subject | ASSAY | - |
dc.subject | DRUG | - |
dc.subject | DNA | - |
dc.title | Opsonized erythrocyte ghosts for liver-targeted delivery of antisense oligodeoxynucleotides | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Yu-Kyoung | - |
dc.identifier.doi | 10.1016/j.biomaterials.2008.10.031 | - |
dc.identifier.scopusid | 2-s2.0-57549118342 | - |
dc.identifier.wosid | 000262552600031 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.30, no.5, pp.959 - 967 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 30 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 959 | - |
dc.citation.endPage | 967 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDES | - |
dc.subject.keywordPlus | PLASMA | - |
dc.subject.keywordPlus | PHOSPHOROTHIOATE | - |
dc.subject.keywordPlus | PHAGOCYTOSIS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | DRUG | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Antisense quantification | - |
dc.subject.keywordAuthor | Antisense delivery | - |
dc.subject.keywordAuthor | Erythrocyte ghost | - |
dc.subject.keywordAuthor | Opsonization | - |
dc.subject.keywordAuthor | Liver targeting | - |
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