Stabilization of Glycoprotein Liquid Formulation Using Arginine: A Study with Lactoferrin as a Model Protein
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun-Jung | - |
dc.contributor.author | Shin, Chang hoon | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.date.accessioned | 2021-09-08T21:04:28Z | - |
dc.date.available | 2021-09-08T21:04:28Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009-01 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120817 | - |
dc.description.abstract | The formulation of new biotherapeutics without human serum albumin (HSA) could decrease the potential risk of blood-transmitted diseases and those caused by infectious viruses and other pathogens. In the present study, arginine was examined as a potential alternative to HAS, and bovine lactoferrin (bLf) was used as a representative model glycoprotein since bLf has potential immunomodulatory and antiviral activity. The optimal formulation for the mixture was determined to be 10 mM arginine, 15% (w/v) trehalose, and 0.02% (v/v) Tween 80, using a statistical analysis program, Minitab. Analyses were performed using reverse-phase high-performance liquid chromatography (HPLC) and SDS-PAGE. The blf HSA-free formulations lost only 12-20% of blf compared with 46% for control (without additives) after 28d of storage. Based on long-term stability studies, the HSA-free formulation developed in this study had a stronger effect on the stability of bLf (1.4-fold) than HSA formulation tinder various storage conditions over 6 months. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | THERMAL-STABILITY | - |
dc.subject | IRON | - |
dc.subject | OPTIMIZATION | - |
dc.subject | TREHALOSE | - |
dc.subject | RELEASE | - |
dc.subject | ALPHA | - |
dc.title | Stabilization of Glycoprotein Liquid Formulation Using Arginine: A Study with Lactoferrin as a Model Protein | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.1271/bbb.80475 | - |
dc.identifier.scopusid | 2-s2.0-58849115460 | - |
dc.identifier.wosid | 000263133400010 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.73, no.1, pp.61 - 66 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.title | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.volume | 73 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 61 | - |
dc.citation.endPage | 66 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | THERMAL-STABILITY | - |
dc.subject.keywordPlus | IRON | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | TREHALOSE | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordAuthor | liquid formulation | - |
dc.subject.keywordAuthor | human serum albumin | - |
dc.subject.keywordAuthor | arginine | - |
dc.subject.keywordAuthor | lactoferrin | - |
dc.subject.keywordAuthor | stabilizing agent | - |
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