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Increased production of S-adenosyl-L-methionine using recombinant Saccharomyces cerevisiae sake K6

Authors
Choi, Eun-SilPark, Bu-SooLee, Sang-WooOh, Min-Kyu
Issue Date
1월-2009
Publisher
KOREAN INSTITUTE CHEMICAL ENGINEERS
Keywords
S-Adenosyl-L-methionine; Saccharomyces cerevisiae Sake; Auxotrophic Mutant; Recombinant DNA
Citation
KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.26, no.1, pp.156 - 159
Indexed
SCIE
SCOPUS
KCI
Journal Title
KOREAN JOURNAL OF CHEMICAL ENGINEERING
Volume
26
Number
1
Start Page
156
End Page
159
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/120886
DOI
10.1007/s11814-009-0025-x
ISSN
0256-1115
Abstract
S. cerevisiae sake K6 was the firstly isolated industrial strain to overproduce S-adenosyl-L-methionine (SAM). Although the strain has advantages over other strains, such as GRAS (generally recognized as safe) property, the S. cerevisiae K6 has not been further developed with DNA recombinant technology due to the lack of a proper genetic marker. In this study, UV mutagenesis was conducted with S. cerevisiae sake K6. With the method, a mutant of sake yeast with leucine auxotroph, K6-1, was isolated. The mutant showed comparable growth rate and SAM productivity with its wild type. Using the auxotroph as a genetic marker, a SAM synthase in S. cerevisiae, SAM2, was overexpressed in the mutant strain. This recombinant DNA technology successfully increased SAM productivity in sake yeast.
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