Microfluidic chip-based electrochemical immunoassay for hippuric acid
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo, Sung Ju | - |
dc.contributor.author | Choi, Young Bong | - |
dc.contributor.author | Il Ju, Jong | - |
dc.contributor.author | Tae, Gun-Sik | - |
dc.contributor.author | Kim, Hyug Han | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.date.accessioned | 2021-09-08T21:23:08Z | - |
dc.date.available | 2021-09-08T21:23:08Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120914 | - |
dc.description.abstract | Urinary hippuric acid (HA), of molecular weight 180 Da, is one of the major metabolites in toluene-exposed humans and is a major biological indicator. Simple and ubiquitous monitoring of exposure to toluene is very important in occupational health care, and a microfluidic chip-based electrochemical immunoassay for rapid and quantitative detection of HA in human urine is proposed in this paper. The system employs a conjugate of ferrocene (Fc) and hippuric acid ( HA). The competition between hippuric acid ( HA) and the ferrocene-hippuric acid complex (Fc-Lys-HA) to bind with a HA antibody coated onto polybeads generated electrical signals proportional to the HA concentration in the range of 0-40 mg mL(-1). All the complicated HA detection processes were integrated on the single microfluidic platform. The quantitative advantages of our HA detection chip are as follows: ( 1) the total chip size was reduced to 3.0 x 2.0 x 0.5 cm and is small enough to be portable, ( 2) the assay time took 1 min, and is shorter than that of conventional electrochemical HA immunoassay systems (about 20 min) and (3) 40 mu L of the sample solution was enough to detect HA in the range of 0-40 mg mL(-1), which is enough range to be used for the point-of-care system. In addition, we suggest the improved chip-based HA assay method by the combination of electrochemical and enzymatic amplification processes for the detection of greater electrical signals. The sensitivity of the combined method was increased about three times compared to that of the non-enzymatic process. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | ENZYME IMMUNOASSAYS | - |
dc.subject | GLUCOSE BIOSENSOR | - |
dc.subject | SYSTEM | - |
dc.subject | MICROCHIP | - |
dc.subject | TOLUENE | - |
dc.subject | URINE | - |
dc.subject | ELECTRODE | - |
dc.subject | DEVICES | - |
dc.subject | IMMUNOGLOBULIN | - |
dc.subject | CHROMATOGRAPHY | - |
dc.title | Microfluidic chip-based electrochemical immunoassay for hippuric acid | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hoon | - |
dc.identifier.doi | 10.1039/b915356j | - |
dc.identifier.scopusid | 2-s2.0-72449157117 | - |
dc.identifier.wosid | 000271864900014 | - |
dc.identifier.bibliographicCitation | ANALYST, v.134, no.12, pp.2462 - 2467 | - |
dc.relation.isPartOf | ANALYST | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 134 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2462 | - |
dc.citation.endPage | 2467 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | ENZYME IMMUNOASSAYS | - |
dc.subject.keywordPlus | GLUCOSE BIOSENSOR | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | MICROCHIP | - |
dc.subject.keywordPlus | TOLUENE | - |
dc.subject.keywordPlus | URINE | - |
dc.subject.keywordPlus | ELECTRODE | - |
dc.subject.keywordPlus | DEVICES | - |
dc.subject.keywordPlus | IMMUNOGLOBULIN | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.