A regenerative electrochemical sensor based on oligonucleotide for the selective determination of mercury(II)
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Donghoon | - |
dc.contributor.author | Kim, Yang-Rae | - |
dc.contributor.author | Oh, Jeong-Wook | - |
dc.contributor.author | Kim, Tae Hyun | - |
dc.contributor.author | Mahajan, Rakesh Kumar | - |
dc.contributor.author | Kim, Jong Seung | - |
dc.contributor.author | Kim, Hasuck | - |
dc.date.accessioned | 2021-09-08T21:29:18Z | - |
dc.date.available | 2021-09-08T21:29:18Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120945 | - |
dc.description.abstract | We have developed a selective, sensitive, and re-usable electrochemical sensor for Hg2+ ion detection. This sensor is based on the Hg2+-induced conformational change of a single-stranded DNA (ssDNA) which involves an electroactive, ferrocene-labeled DNA hairpin structure and provides strategically the selective binding of a thymine-thymine mismatch for the Hg2+ ion. The ferrocene-labeled DNA is self-assembled through S-Au bonding on a polycrystalline gold electrode surface and the surface blocked with 3-mercapto-1-propanol to form a mixed monolayer. The modified electrode showed a voltammetric signal due to a one-step redox reaction of the surface-confined ferrocenyl moiety. The `signal-on' upon mercury binding could be attributed to a change in the conformation of ferrocene-labeled DNA from an open structure to a restricted hairpin structure. The differential pulse voltammetry (DPV) of the modified electrode showed a linear response of the ferrocene oxidation signal with increase of Hg2+ concentration in the range between 0.1 and 2 mu M with a detection limit of 0.1 mM. The molecular beacon mercury(II) ion sensor was amenable to regeneration by simply unfolding the ferrocene-labeled DNA in 10 mu Mcysteine, and could be regenerated with no loss in signal gain upon subsequent mercury(II) ion binding. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | ANODIC-STRIPPING VOLTAMMETRY | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | ELECTRON-TRANSFER | - |
dc.subject | FERROCENE DERIVATIVES | - |
dc.subject | AQUEOUS-SOLUTION | - |
dc.subject | DNA MONOLAYERS | - |
dc.subject | SOIL SAMPLES | - |
dc.subject | HG-II | - |
dc.subject | GOLD | - |
dc.subject | ENZYME | - |
dc.title | A regenerative electrochemical sensor based on oligonucleotide for the selective determination of mercury(II) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jong Seung | - |
dc.identifier.doi | 10.1039/b908457f | - |
dc.identifier.scopusid | 2-s2.0-70349337804 | - |
dc.identifier.wosid | 000269063100017 | - |
dc.identifier.bibliographicCitation | ANALYST, v.134, no.9, pp.1857 - 1862 | - |
dc.relation.isPartOf | ANALYST | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 134 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1857 | - |
dc.citation.endPage | 1862 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | ANODIC-STRIPPING VOLTAMMETRY | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | ELECTRON-TRANSFER | - |
dc.subject.keywordPlus | FERROCENE DERIVATIVES | - |
dc.subject.keywordPlus | AQUEOUS-SOLUTION | - |
dc.subject.keywordPlus | DNA MONOLAYERS | - |
dc.subject.keywordPlus | SOIL SAMPLES | - |
dc.subject.keywordPlus | HG-II | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | ENZYME | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.