초고압전자현미경을 이용한 초파리 망막 세포의 3차원 구조Three Dimensional Reconstruction of Cellular Structure in Drosophila Retina Using High Voltage Electron Microscopy
- Other Titles
- Three Dimensional Reconstruction of Cellular Structure in Drosophila Retina Using High Voltage Electron Microscopy
- Authors
- 문지영; 이경은; 한성식
- Issue Date
- 2009
- Publisher
- 한국현미경학회
- Keywords
- HVEM; 3D structure. Retina; Drosophila melanogaster
- Citation
- 한국현미경학회지, v.39, no.2, pp.185 - 189
- Indexed
- KCI
- Journal Title
- 한국현미경학회지
- Volume
- 39
- Number
- 2
- Start Page
- 185
- End Page
- 189
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/121017
- ISSN
- 2287-5123
- Abstract
- Studies about the structure of Drosophila melanogaster retinal cell using electron microscopy have been carried out in
details since 1960s. However, these results can have limitations in functional research because of two-dimensional
structure. In this study, the adult retina of Drosophila melanogaster was investigated by employing high pressure freezing
method, serial sections, high voltage electron microscopy, and 3-dimensional reconstruction method. From there results,
mitochondria, microtubules, and nuclei were reconstructed as 3-dimensional structure using IMOD program. The 3D
structure of these organelles showed that mitochondira mainly located in distal region near lens, and microtubule mainly
located in distal and basal region. The 3D reconstruction of these organelles can be used for a critical evaluation in the
dynamic change of cellular organelles caused by functional abnormality like retinal degeneration.
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