A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter ActivityA New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity
- Other Titles
- A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity
- Authors
- 정선도; 최지혜; 홍창완; 이현지; 박윤경; 신정훈; 박재원; 박세호
- Issue Date
- 2009
- Publisher
- 대한면역학회
- Keywords
- dual reporter vector system; transcription activity; promoter assay; FACS
- Citation
- Immune Network, v.9, no.6, pp.243 - 247
- Indexed
- KCI
- Journal Title
- Immune Network
- Volume
- 9
- Number
- 6
- Start Page
- 243
- End Page
- 247
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/121575
- ISSN
- 1598-2629
- Abstract
- In this study, we report the development of a new dual reporter
vector system for the analysis of promoter activity.
This system employs green fluorescence emitting protein,
EGFP, as a reporter, and uses red fluorescence emitting protein,
DsRed, as a transfection control in a single vector. The
expression of those two proteins can be readily detected via
flow cytometry in a single analysis, with no need for any further
manipulation after transfection. As this system allows
for the simultaneous detection of both the control and reporter
proteins in the same cells, only transfected cells which
express the control protein, DsRed, can be subjected to promoter
activity analysis, via the gating out of all un-transfected
cells. This results in a dramatic increase in the promoter
activity detection sensitivity. This novel reporter vector
system should prove to be a simple and efficient method
for the analysis of promoter activity.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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