Enhanced cardiomyogenic differentiation of P19 embryonal carcinoma stem cells
DC Field | Value | Language |
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dc.contributor.author | Yang, J. | - |
dc.contributor.author | Ko, S.-J. | - |
dc.contributor.author | Kim, B.-S. | - |
dc.contributor.author | Kim, H.-S. | - |
dc.contributor.author | Park, S. | - |
dc.contributor.author | Hong, D. | - |
dc.contributor.author | Hong, S.-W. | - |
dc.contributor.author | Choi, J.-H. | - |
dc.contributor.author | Park, C.-Y. | - |
dc.contributor.author | Choi, S.-C. | - |
dc.contributor.author | Hong, S.-J. | - |
dc.contributor.author | Lim, D.-S. | - |
dc.date.accessioned | 2021-09-09T00:13:51Z | - |
dc.date.available | 2021-09-09T00:13:51Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1738-5520 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/121852 | - |
dc.description.abstract | Background and Objectives: We investigated the effects of different concentrations of serum, 5-azacytidine, and culture time on the cardiomyogenic differentiation of P19 embryonal carcinoma stem cells in the course of developing an efficient protocol for generating the cardiomyogenic lineage. Materials and Methods: P19 cells were plated at a density of 1 × 10 6 cells on 10-cm bacterial dishes for 96 hours in the presence of 1% dimethyl sulfoxide to form embryoid bodies. The embryoid bodies were cultured in medium with 2% or 10% fetal bovine serum for an additional 10 or 15 consecutive days in the presence of 0, 1, or 3 μM 5-azacytidine. Results: Quantitative real-time polymerase chain reaction (PCR) analysis showed that the messenger ribonucleic acid (mRNA) expression of cardiac muscle-specific genes, such as GATA4, α-actin, α-myosin heavy chain, and cardiac troponin T, were significantly higher in the 15-day culture groups than in the 10-day culture groups. Furthermore, the cardiac muscle-specific genes were expressed more in the high-serum groups compared to the low-serum groups regardless of the culture time. Cardiomyogenic differentiation of the P19 cells was most effective in 1 μM 5-aza-cytidine regardless of the serum concentrations. In addition, the stimulation effects of 5-azacytidine on cardiomyogenic differentiation were more significant under low-serum culture conditions compared to high-serum culture conditions. Cardiomyogenic differentiation of P19 cells was further confirmed by immunostaining with cardiac muscle-specific antibodies. Conclusion: Taken together, these results demonstrated that cardiomyogenic differentiation of P19 cells was enhanced by a combination of different experimental factors. Copyright © 2009 The Korean Society of Cardiology. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.subject | alpha actin | - |
dc.subject | antibody | - |
dc.subject | azacitidine | - |
dc.subject | dimethyl sulfoxide | - |
dc.subject | messenger RNA | - |
dc.subject | myosin heavy chain alpha | - |
dc.subject | transcription factor GATA 4 | - |
dc.subject | troponin T | - |
dc.subject | animal cell | - |
dc.subject | article | - |
dc.subject | bacterium | - |
dc.subject | blood level | - |
dc.subject | cell culture | - |
dc.subject | cell density | - |
dc.subject | cell differentiation | - |
dc.subject | cell lineage | - |
dc.subject | cell stimulation | - |
dc.subject | controlled study | - |
dc.subject | cow | - |
dc.subject | culture medium | - |
dc.subject | embryo | - |
dc.subject | embryonal carcinoma stem cell | - |
dc.subject | gene | - |
dc.subject | gene expression | - |
dc.subject | heart muscle | - |
dc.subject | immunohistochemistry | - |
dc.subject | mouse | - |
dc.subject | nonhuman | - |
dc.subject | polymerase chain reaction | - |
dc.subject | quantitative analysis | - |
dc.subject | serum | - |
dc.title | Enhanced cardiomyogenic differentiation of P19 embryonal carcinoma stem cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, S.-C. | - |
dc.identifier.doi | 10.4070/kcj.2009.39.5.198 | - |
dc.identifier.scopusid | 2-s2.0-67549119402 | - |
dc.identifier.bibliographicCitation | Korean Circulation Journal, v.39, no.5, pp.198 - 204 | - |
dc.relation.isPartOf | Korean Circulation Journal | - |
dc.citation.title | Korean Circulation Journal | - |
dc.citation.volume | 39 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 198 | - |
dc.citation.endPage | 204 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001343808 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | alpha actin | - |
dc.subject.keywordPlus | antibody | - |
dc.subject.keywordPlus | azacitidine | - |
dc.subject.keywordPlus | dimethyl sulfoxide | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | myosin heavy chain alpha | - |
dc.subject.keywordPlus | transcription factor GATA 4 | - |
dc.subject.keywordPlus | troponin T | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | bacterium | - |
dc.subject.keywordPlus | blood level | - |
dc.subject.keywordPlus | cell culture | - |
dc.subject.keywordPlus | cell density | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell lineage | - |
dc.subject.keywordPlus | cell stimulation | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | cow | - |
dc.subject.keywordPlus | culture medium | - |
dc.subject.keywordPlus | embryo | - |
dc.subject.keywordPlus | embryonal carcinoma stem cell | - |
dc.subject.keywordPlus | gene | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | heart muscle | - |
dc.subject.keywordPlus | immunohistochemistry | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | polymerase chain reaction | - |
dc.subject.keywordPlus | quantitative analysis | - |
dc.subject.keywordPlus | serum | - |
dc.subject.keywordAuthor | Cell differentiation | - |
dc.subject.keywordAuthor | Embryonal carcinoma stem cells | - |
dc.subject.keywordAuthor | Myocytes, cardiac | - |
dc.subject.keywordAuthor | Serum | - |
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