SMD and NMD are competitive pathways that contribute to myogenesis: Effects on PAX3 and myogenin mRNAs
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gong, C. | - |
dc.contributor.author | Kim, Y.K. | - |
dc.contributor.author | Woeller, C.F. | - |
dc.contributor.author | Tang, Y. | - |
dc.contributor.author | Maquat, L.E. | - |
dc.date.accessioned | 2021-09-09T00:34:27Z | - |
dc.date.available | 2021-09-09T00:34:27Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0890-9369 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/121940 | - |
dc.description.abstract | UPF1 functions in both Staufen 1 (STAU1)-mediated mRNA decay (SMD) and nonsense-mediated mRNA decay (NMD), which we show here are competitive pathways. STAU1- and UPF2-binding sites within UPF1 overlap so that STAU1 and UPF2 binding to UPF1 appear to be mutually exclusive. Furthermore, down-regulating the cellular abundance of STAU1, which inhibits SMD, increases the efficiency of NMD, whereas down-regulating the cellular abundance of UPF2, which inhibits NMD, increases the efficiency of SMD. Competition under physiological conditions is exemplified during the differentiation of C2C12 myoblasts to myotubes: The efficiency of SMD increases and the efficiency of NMD decreases, consistent with our finding that more STAU1 but less UPF2 bind UPF1 in myotubes compared with myoblasts. Moreover, an increase in the cellular level of UPF3X during myogenesis results in an increase in the efficiency of an alternative NMD pathway that, unlike classical NMD, is largely insensitive to UPF2 down-regulation. We discuss the remarkable balance between SMD and the two types of NMD in view of data indicating that PAX3 mRNA is an SMD target whose decay promotes myogenesis whereas myogenin mRNA is a classical NMD target encoding a protein required for myogenesis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.subject | cell protein | - |
dc.subject | messenger RNA | - |
dc.subject | myogenin | - |
dc.subject | protei upf2 | - |
dc.subject | protein staufen 1 | - |
dc.subject | protein Upf1 | - |
dc.subject | transcription factor PAX3 | - |
dc.subject | unclassified drug | - |
dc.subject | animal cell | - |
dc.subject | article | - |
dc.subject | cell differentiation | - |
dc.subject | cell level | - |
dc.subject | controlled study | - |
dc.subject | down regulation | - |
dc.subject | human | - |
dc.subject | human cell | - |
dc.subject | muscle development | - |
dc.subject | myoblast | - |
dc.subject | myotube | - |
dc.subject | nonhuman | - |
dc.subject | nonsense mediated mRNA decay | - |
dc.subject | priority journal | - |
dc.subject | protein binding | - |
dc.subject | RNA degradation | - |
dc.subject | staufen 1 mediated mRNA decay | - |
dc.subject | Amino Acids | - |
dc.subject | Animals | - |
dc.subject | Binding Sites | - |
dc.subject | Cell Differentiation | - |
dc.subject | Cell Line | - |
dc.subject | Cercopithecus aethiops | - |
dc.subject | COS Cells | - |
dc.subject | Cytoskeletal Proteins | - |
dc.subject | Down-Regulation | - |
dc.subject | Hela Cells | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Microtubules | - |
dc.subject | Muscle Development | - |
dc.subject | Myoblasts | - |
dc.subject | Myogenin | - |
dc.subject | Paired Box Transcription Factors | - |
dc.subject | RNA Stability | - |
dc.subject | RNA, Messenger | - |
dc.subject | RNA-Binding Proteins | - |
dc.subject | Trans-Activators | - |
dc.title | SMD and NMD are competitive pathways that contribute to myogenesis: Effects on PAX3 and myogenin mRNAs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Y.K. | - |
dc.identifier.doi | 10.1101/gad.1717309 | - |
dc.identifier.scopusid | 2-s2.0-58149520665 | - |
dc.identifier.bibliographicCitation | Genes and Development, v.23, no.1, pp.54 - 66 | - |
dc.relation.isPartOf | Genes and Development | - |
dc.citation.title | Genes and Development | - |
dc.citation.volume | 23 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 54 | - |
dc.citation.endPage | 66 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | cell protein | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | myogenin | - |
dc.subject.keywordPlus | protei upf2 | - |
dc.subject.keywordPlus | protein staufen 1 | - |
dc.subject.keywordPlus | protein Upf1 | - |
dc.subject.keywordPlus | transcription factor PAX3 | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell level | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | down regulation | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | muscle development | - |
dc.subject.keywordPlus | myoblast | - |
dc.subject.keywordPlus | myotube | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | nonsense mediated mRNA decay | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein binding | - |
dc.subject.keywordPlus | RNA degradation | - |
dc.subject.keywordPlus | staufen 1 mediated mRNA decay | - |
dc.subject.keywordPlus | Amino Acids | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Binding Sites | - |
dc.subject.keywordPlus | Cell Differentiation | - |
dc.subject.keywordPlus | Cell Line | - |
dc.subject.keywordPlus | Cercopithecus aethiops | - |
dc.subject.keywordPlus | COS Cells | - |
dc.subject.keywordPlus | Cytoskeletal Proteins | - |
dc.subject.keywordPlus | Down-Regulation | - |
dc.subject.keywordPlus | Hela Cells | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Mice | - |
dc.subject.keywordPlus | Microtubules | - |
dc.subject.keywordPlus | Muscle Development | - |
dc.subject.keywordPlus | Myoblasts | - |
dc.subject.keywordPlus | Myogenin | - |
dc.subject.keywordPlus | Paired Box Transcription Factors | - |
dc.subject.keywordPlus | RNA Stability | - |
dc.subject.keywordPlus | RNA, Messenger | - |
dc.subject.keywordPlus | RNA-Binding Proteins | - |
dc.subject.keywordPlus | Trans-Activators | - |
dc.subject.keywordAuthor | Myogenesis | - |
dc.subject.keywordAuthor | Nonsense-mediated mrna decay | - |
dc.subject.keywordAuthor | Premature termination codon | - |
dc.subject.keywordAuthor | Staufen1 | - |
dc.subject.keywordAuthor | Staufen1-mediated mrna decay | - |
dc.subject.keywordAuthor | Upf proteins | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.