Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability
DC Field | Value | Language |
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dc.contributor.author | Kim, Choong | - |
dc.contributor.author | Lee, Kang Sun | - |
dc.contributor.author | Kim, Young Eun | - |
dc.contributor.author | Lee, Kyu-Jung | - |
dc.contributor.author | Lee, Soo Hyun | - |
dc.contributor.author | Kim, Tae Song | - |
dc.contributor.author | Kang, Ji Yoon | - |
dc.date.accessioned | 2021-09-09T01:04:21Z | - |
dc.date.available | 2021-09-09T01:04:21Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122114 | - |
dc.description.abstract | This paper describes a microfluidic device for the microencapsulation of cells in alginate beads to enhance cell viability. The alginate droplet including cells was gelified with calcified oleic acid, using two-phase microfluidics. The on-chip gelation had generated monodisperse spherical alginate beads, which could not be readily obtained via conventional external gelation in a calcium chloride bath. However, the prolonged exposure of encapsulated cells to the toxic oil phase caused serious damage to the cells. Therefore, we proposed the formulation of a rapid oil-exchange chip which transforms the toxic oleic acid to harmless mineral oil. The flushing out of oleic acid after the gelation of alginate beads effected a dramatic increase in the viability of P19 embryonic carcinoma cells, up to 90%. The experimental results demonstrated that the cell viability was proportional to the flow rate of squeezing mineral oil. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | MICROFLUIDIC DEVICE | - |
dc.subject | ENCAPSULATION | - |
dc.subject | MICROBEADS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | MICROSPHERES | - |
dc.title | Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kyu-Jung | - |
dc.identifier.doi | 10.1039/b819044e | - |
dc.identifier.scopusid | 2-s2.0-65349196129 | - |
dc.identifier.wosid | 000265223200022 | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.9, no.9, pp.1294 - 1297 | - |
dc.relation.isPartOf | LAB ON A CHIP | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 9 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1294 | - |
dc.citation.endPage | 1297 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICE | - |
dc.subject.keywordPlus | ENCAPSULATION | - |
dc.subject.keywordPlus | MICROBEADS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MICROSPHERES | - |
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