A Metabonomic Study on the Biochemical Effects of Doxorubicin in Rats Using 1H-NMR Spectroscopy
DC Field | Value | Language |
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dc.contributor.author | Park, Jong-Chul | - |
dc.contributor.author | Hong, Young-Shick | - |
dc.contributor.author | Kim, Yeon Joo | - |
dc.contributor.author | Yang, Ji-Young | - |
dc.contributor.author | Kim, Eun-Young | - |
dc.contributor.author | Kwack, Seung Jun | - |
dc.contributor.author | Ryu, Do Hyun | - |
dc.contributor.author | Hwang, Geum-Sook | - |
dc.contributor.author | Lee, Byung Mu | - |
dc.date.accessioned | 2021-09-09T01:06:25Z | - |
dc.date.available | 2021-09-09T01:06:25Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1528-7394 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122125 | - |
dc.description.abstract | Metabonomic investigation of doxorubicin (adriamycin) was carried out in male Sprague-Dawley rats using high-resolution 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate statistics. Urine samples (d -1 to 7) from rats treated with doxorubicin at two dose levels (5 or 15 mg/kg body weight) were collected at each time point and doxorubicin-induced biomarkers were examined. Of metabolites, early elevated biochemical changes were observed in trimethylamine N-oxide (TMAO) levels suggesting renal dysfunction. Perturbation in TMAO was maximal in the low-dose group at 48 h post dose (p.d.) and returned to control at 168 h p.d., indicating recovery from renal toxicity induced by doxorubicin. After doxorubicin administration, the high-dose group was divided into low and high responders at 48 h and further divided into high, moderate, and no recovery animals at 96 h, indicating individual susceptible response to drug-induced toxicity. Urinary increases in glucose, lactate, alanine, and valine suggested progression of renal damage resulting in glycosuria, lactic aciduria, and aminoaciduria up to 168 h in the high-dose group. Urinary elevation of creatine and phenylacetylglycine (PAG) together with reduction of N-methylnicotinic acid (NMNA) and hippurate levels was suggestive of liver injury in the high-dose group. Impairment of energy metabolism was also indicated by decreased levels of tricarboxylic acid cycle intermediates in urine of rats treated with high-dose doxorubicin. This study highlights the applicability of NMR-based metabonomics with multivariate statistics for monitoring biomarkers produced by doxorubicin treatments. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.subject | GLYCINE CONJUGATION | - |
dc.subject | BENZOIC-ACID | - |
dc.subject | HYDRAZINE TOXICITY | - |
dc.subject | LIPID-PEROXIDATION | - |
dc.subject | NMR-SPECTROSCOPY | - |
dc.subject | CREATINE-KINASE | - |
dc.subject | RENAL DAMAGE | - |
dc.subject | URINARY | - |
dc.subject | LIVER | - |
dc.subject | METABOLISM | - |
dc.title | A Metabonomic Study on the Biochemical Effects of Doxorubicin in Rats Using 1H-NMR Spectroscopy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Young-Shick | - |
dc.identifier.doi | 10.1080/15287390802647195 | - |
dc.identifier.scopusid | 2-s2.0-60549096668 | - |
dc.identifier.wosid | 000263143500003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.72, no.6, pp.374 - 384 | - |
dc.relation.isPartOf | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | - |
dc.citation.title | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | - |
dc.citation.volume | 72 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 374 | - |
dc.citation.endPage | 384 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalResearchArea | Public, Environmental & Occupational Health | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.relation.journalWebOfScienceCategory | Public, Environmental & Occupational Health | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | GLYCINE CONJUGATION | - |
dc.subject.keywordPlus | BENZOIC-ACID | - |
dc.subject.keywordPlus | HYDRAZINE TOXICITY | - |
dc.subject.keywordPlus | LIPID-PEROXIDATION | - |
dc.subject.keywordPlus | NMR-SPECTROSCOPY | - |
dc.subject.keywordPlus | CREATINE-KINASE | - |
dc.subject.keywordPlus | RENAL DAMAGE | - |
dc.subject.keywordPlus | URINARY | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | METABOLISM | - |
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