Oxidative stress induces PKR-dependent apoptosis via IFN-gamma activation signaling in Jurkat T cells
DC Field | Value | Language |
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dc.contributor.author | Pyo, Chul-Woong | - |
dc.contributor.author | Lee, Shin-Hee | - |
dc.contributor.author | Choi, Sang-Yun | - |
dc.date.accessioned | 2021-09-09T01:25:48Z | - |
dc.date.available | 2021-09-09T01:25:48Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-12-19 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122205 | - |
dc.description.abstract | The dsRNA-dependent protein kinase, PKR, is a central component in antiviral defense. The biological importance of PKR is further remarked by its critical role in apoptosis induced by a variety of stresses. Here, we analyzed the implication of oxidative stress in the induction of PKR-dependent apoptosis in Jurkat cells. Our results revealed that reactive oxygen species (ROS) induced endogenous pkr gene expression at the transcriptional level by activating the interferon (IFN)-gamma gene. However, IFN-gamma siRNA expression abrogated the H2O2-mediated pkr induction. The radical scavenger N-acetyl-L-cysteine profoundly inhibited pkr induction via the reduction of IFN-gamma expression. The treatment of cells with the specific JAK-STAT inhibitor, AG490, reduced the PKR expression, and Suppressed PKR-dependent cell death. Finally, siRNA-mediated depletion of IFN-gamma or pkr efficiently downregulated H2O2-mediated apoptotic cell death. These results indicated that oxidative stress induces PKR expression essentially via the IFN-gamma activation signal, and causes apoptosis in Jurkat T cells. (C) 2008 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | PROTEIN-KINASE PKR | - |
dc.subject | HIV-1 TAT | - |
dc.subject | INTERFERON | - |
dc.subject | PROMOTER | - |
dc.subject | MECHANISMS | - |
dc.subject | COMPLEX | - |
dc.title | Oxidative stress induces PKR-dependent apoptosis via IFN-gamma activation signaling in Jurkat T cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Sang-Yun | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.10.103 | - |
dc.identifier.scopusid | 2-s2.0-56149097715 | - |
dc.identifier.wosid | 000261458900051 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.377, no.3, pp.1001 - 1006 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 377 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1001 | - |
dc.citation.endPage | 1006 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | PROTEIN-KINASE PKR | - |
dc.subject.keywordPlus | HIV-1 TAT | - |
dc.subject.keywordPlus | INTERFERON | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | Interferon | - |
dc.subject.keywordAuthor | PKR | - |
dc.subject.keywordAuthor | Apoptosis | - |
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