Optimization of the Process for Biodiesel Production Using a Mixture of Immobilized Rhizopus oryzae and Candida rugosa Lipases
- Authors
- Lee, Jong Ho; Lee, Dong Hwan; Lim, Jung Soo; Um, Byung-Hwan; Park, Chulhwan; Kang, Seong Woo; Kim, Seung Wook
- Issue Date
- 12월-2008
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Biodiesel; immobilization; Rhizopus oryzae lipase; Candida rugosa lipase
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.12, pp.1927 - 1931
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 12
- Start Page
- 1927
- End Page
- 1931
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122267
- DOI
- 10.4014/jmb.0800.054
- ISSN
- 1017-7825
- Abstract
- In this study, the enzymatic process for biodiesel production was optimized using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. The optimal temperature and agitation speed for biodiesel production were 45 degrees C and 300 rpm, respectively. The optimal ratio of R. oryzae and C. rugosa lipases in the mixture was 3:1 (w:w). When 3 mmol of methanol was the initial reaction medium and 3 mmol of methanol was added every 1.5 h during biodiesel production, biodiesel conversion was over 98% at 4 h. In addition, when the immobilized lipase mixture was reused, biodiesel conversion exceeded 80% after 5 reuses.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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