Crystallization and preliminary X-ray crystallographic studies of the rho-class glutathione S-transferase from the Antarctic clam Laternula elliptica
DC Field | Value | Language |
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dc.contributor.author | Jang, Eun Hyuk | - |
dc.contributor.author | Park, Hyun | - |
dc.contributor.author | Park, Ae Kyung | - |
dc.contributor.author | Moon, Jin Ho | - |
dc.contributor.author | Chi, Young Min | - |
dc.contributor.author | Ahn, In Young | - |
dc.date.accessioned | 2021-09-09T02:09:03Z | - |
dc.date.available | 2021-09-09T02:09:03Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-12 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122330 | - |
dc.description.abstract | Glutathione S-transferases are involved in phase II detoxification processes and catalyze the nucleophilic attack of the tripeptide glutathione on a wide range of endobiotic and xenobiotic electrophilic substrates. The rho-class glutathione S-transferase from Laternula elliptica was overexpressed in Escherichia coli, purified and crystallized with two substrates: glutathione and 1-chloro-2,4-dinitrobenzene (CDNB). Diffraction data were collected to 2.20 angstrom resolution for the glutathione-complex crystals and to 2.00 angstrom resolution for the CDNB-complex crystals using a synchrotron-radiation source. Both crystals belonged to the C-centred monoclinic space group C2. The unit-cell parameters for the CDNB-complex crystals were a = 89.66, b = 59.27, c = 55.45 angstrom, beta = 124.52 degrees. The asymmetric unit contained one molecule, with a corresponding V-M of 2.36 angstrom(3) Da(-1) and a solvent content of 47.8%. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | ELECTROPHILE BINDING-SITE | - |
dc.subject | EXPRESSION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | MODE | - |
dc.title | Crystallization and preliminary X-ray crystallographic studies of the rho-class glutathione S-transferase from the Antarctic clam Laternula elliptica | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Hyun | - |
dc.contributor.affiliatedAuthor | Moon, Jin Ho | - |
dc.contributor.affiliatedAuthor | Chi, Young Min | - |
dc.identifier.doi | 10.1107/S1744309108034003 | - |
dc.identifier.scopusid | 2-s2.0-57349150773 | - |
dc.identifier.wosid | 000261198700012 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.64, pp.1132 - 1134 | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.volume | 64 | - |
dc.citation.startPage | 1132 | - |
dc.citation.endPage | 1134 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | ELECTROPHILE BINDING-SITE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | MODE | - |
dc.subject.keywordAuthor | ρ class | - |
dc.subject.keywordAuthor | CDNB | - |
dc.subject.keywordAuthor | Glutathione | - |
dc.subject.keywordAuthor | Glutathione S-transferases | - |
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