Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Structural studies of human brain-type creatine kinase complexed with the ADP-Mg2+-NO3--creatine transition-state analogue complex

Authors
Bong, Seoung MinMoon, Jin HoNam, Ki HyunLee, Ki SeogChi, Young MinHwang, Kwang Yeon
Issue Date
26-11월-2008
Publisher
WILEY
Keywords
Brain-type creatine kinase; Shuttle system; Energy homeostasis; Crystal structure; Creatine complex
Citation
FEBS LETTERS, v.582, no.28, pp.3959 - 3965
Indexed
SCIE
SCOPUS
Journal Title
FEBS LETTERS
Volume
582
Number
28
Start Page
3959
End Page
3965
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/122383
DOI
10.1016/j.febslet.2008.10.039
ISSN
0014-5793
Abstract
Creatine kinase is a member of the phosphagen kinase family, which catalyzes the reversible phosphoryl transfer reaction that occurs between ATP and creatine to produce ADP and phosphocreatine. Here, three structural aspects of human-brain-type-creatine-kinase (hBB-CK) were identified by X-ray crystallography: the ligand-free-form at 2.2 angstrom; the ADP-Mg2+, nitrate, and creatine complex (transition-state-analogue complex; TSAC); and the ADP-Mg2+-complex at 2.0 angstrom. The structures of ligand-bound hBB-CK revealed two different monomeric states in a single homodimer. One monomer is a closed form, either bound to TSAC or the ADP-Mg2+-complex, and the second monomer is an unliganded open form. These structural studies provide a detailed mechanism indicating that the binding of ADP-Mg2+ alone may trigger conformational changes in hBB-CK that were not observed with muscle-type-CK. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biosystems and Biotechnology > 1. Journal Articles
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Chi, Young Min photo

Chi, Young Min
생명과학대학 (생명공학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE