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Expression Profiling of Streptomyces peucetius Metabolic Genes Using DNA Microarray Analysis

Authors
Yang, Joon-RyeolSong, EunjungKim, Byung-GeeKim, Eung-SooSohng, Jae-KyungOh, Min-Kyu
Issue Date
11월-2008
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Keywords
Streptomyces peucetius; DNA microarray; doxorubicin; metabolic gene
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.6, pp.738 - 744
Indexed
SCIE
SCOPUS
KCI
Journal Title
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume
13
Number
6
Start Page
738
End Page
744
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/122446
DOI
10.1007/s12257-008-0114-4
ISSN
1226-8372
Abstract
Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR. (C) KSBB
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