Improved DNA Chip with Poly(amidoamine) Dendrimer Peripherally Modified with Biotin and Avidin
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bin Lim, Sung | - |
dc.contributor.author | Kim, Kyung-Woo | - |
dc.contributor.author | Lee, Chan-Woo | - |
dc.contributor.author | Kim, Hyo-Sun | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-09T02:54:33Z | - |
dc.date.available | 2021-09-09T02:54:33Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122448 | - |
dc.description.abstract | Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity. (C) KSBB | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | SIGNAL AMPLIFICATION | - |
dc.subject | PROTEIN MICROARRAYS | - |
dc.subject | SENSITIVITY | - |
dc.subject | BINDING | - |
dc.subject | SAMPLES | - |
dc.subject | GOLD | - |
dc.title | Improved DNA Chip with Poly(amidoamine) Dendrimer Peripherally Modified with Biotin and Avidin | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1007/s12257-008-0055-y | - |
dc.identifier.scopusid | 2-s2.0-59849110207 | - |
dc.identifier.wosid | 000262428600006 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.6, pp.683 - 689 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 13 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 683 | - |
dc.citation.endPage | 689 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001300720 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | SIGNAL AMPLIFICATION | - |
dc.subject.keywordPlus | PROTEIN MICROARRAYS | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | SAMPLES | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordAuthor | PAMAM dendrimer | - |
dc.subject.keywordAuthor | DNA chip | - |
dc.subject.keywordAuthor | fluorescence | - |
dc.subject.keywordAuthor | avidin-biotin conjugation | - |
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