Improved serum stability and biophysical properties of siRNAs following chemical modifications
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Ik Sang | - |
dc.contributor.author | Kim, Jung | - |
dc.contributor.author | Lim, Do Hwan | - |
dc.contributor.author | Ahn, Hee-Chul | - |
dc.contributor.author | Kim, Hyunggee | - |
dc.contributor.author | Lee, Kang-Bong | - |
dc.contributor.author | Lee, Young Sik | - |
dc.date.accessioned | 2021-09-09T02:56:06Z | - |
dc.date.available | 2021-09-09T02:56:06Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122456 | - |
dc.description.abstract | Small interfering RNAs (siRNAs) which mediate sequence-specific gene silencing through RNA interference have been harnessed for therapeutic applications. Here, we found combinations of modest 2'-ribose siRNA modifications that permit effective gene silencing and improve siRNA stability in human serum. Introduction of as few as two different or identical 2'-ribose modifications at a specific position of either strand, which has been previously suggested to suppress unintended off-target effects, enhances the thermal and thermodynamic stability of siRNA duplexes, and does not alter the overall A-form helical geometry that is required for silencing activity. Our results provide a useful template for the design for therapeutic siRNAs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | SMALL INTERFERING RNA | - |
dc.subject | FUNCTIONAL FEATURES | - |
dc.subject | STRUCTURAL BASIS | - |
dc.subject | DEGRADATION | - |
dc.subject | RECOGNITION | - |
dc.title | Improved serum stability and biophysical properties of siRNAs following chemical modifications | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunggee | - |
dc.contributor.affiliatedAuthor | Lee, Young Sik | - |
dc.identifier.doi | 10.1007/s10529-008-9776-4 | - |
dc.identifier.scopusid | 2-s2.0-52449103310 | - |
dc.identifier.wosid | 000259412200004 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.30, no.11, pp.1901 - 1908 | - |
dc.relation.isPartOf | BIOTECHNOLOGY LETTERS | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 30 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1901 | - |
dc.citation.endPage | 1908 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | SMALL INTERFERING RNA | - |
dc.subject.keywordPlus | FUNCTIONAL FEATURES | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordAuthor | A-form helix | - |
dc.subject.keywordAuthor | melting temperature | - |
dc.subject.keywordAuthor | 2 &apos | - |
dc.subject.keywordAuthor | -ribose modification | - |
dc.subject.keywordAuthor | RNA interference | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | stability | - |
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