Effects of Leptin on Lipid Metabolism and Gene Expression of Differentiation-Associated Growth Factors and Transcription Factors during Differentiation and Maturation of 3T3-L1 Preadipocytes
DC Field | Value | Language |
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dc.contributor.author | Kim, Won Kon | - |
dc.contributor.author | Lee, Chul Young | - |
dc.contributor.author | Kang, Moon Sung | - |
dc.contributor.author | Kim, Min Ho | - |
dc.contributor.author | Ryu, Yang Hwan | - |
dc.contributor.author | Bae, Kwang-Hee | - |
dc.contributor.author | Shin, Sung Jae | - |
dc.contributor.author | Lee, Sang Chul | - |
dc.contributor.author | Ko, Yong | - |
dc.date.accessioned | 2021-09-09T03:52:36Z | - |
dc.date.available | 2021-09-09T03:52:36Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-10 | - |
dc.identifier.issn | 0918-8959 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122610 | - |
dc.description.abstract | The present study was designed to determine the effects of leptin on lipid metabolism and gene expression during differentiation and maturation of the 3T3-L1 murine preadipocyte. The preadipocytes were induced to differentiate in a growth medium containing 10% calf serum and a hormonal cocktail for 2 days. The cells were next allowed to maturate for 14 days in the growth medium supplemented with 10 mu g/ml insulin or 500 ng/ml insulin-like growth factor (IGF)-I in the absence or presence of supplemented leptin. Leptin, at a dose of 5 to 500 ng/ml, had no effect on proliferation of undifferentiated 3T3-L1 cells. However, leptin suppressed the insulin- or IGF-I-stimulated lipid accumulation and enhanced the release of glycerol, a measure of lipolysis, in a dose-dependent manner during and after the maturation of the cell. Moreover, leptin at a dose of 50 ng/ml inhibited IGF-I gene expression during the entire differentiation and maturation and also peroxisome proliferator activated receptor (PPAR)-gamma expression during late maturation as monitored by semi-quantitative reverse transcription-polymerase chain reaction. However, leptin exerted no effect on the expression of transforming growth factor-beta, CCAT/enhancer binding protein-alpha and PPAR-delta. Taken together, results Suggest the anti-lipogenic and lipolytic effects of leptin in differentiating and mature adipocytes may have been partly mediated by suppressing the expression of PPAR-gamma and IGF-I genes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | JAPAN ENDOCRINE SOC | - |
dc.subject | ADIPOCYTE DIFFERENTIATION | - |
dc.subject | IN-VITRO | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | PROLIFERATION | - |
dc.subject | PROTEIN | - |
dc.subject | BETA | - |
dc.subject | INHIBITION | - |
dc.subject | ENDOCRINE | - |
dc.subject | RECEPTOR | - |
dc.subject | TISSUE | - |
dc.title | Effects of Leptin on Lipid Metabolism and Gene Expression of Differentiation-Associated Growth Factors and Transcription Factors during Differentiation and Maturation of 3T3-L1 Preadipocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Yong | - |
dc.identifier.doi | 10.1507/endocrj.K08E-115 | - |
dc.identifier.scopusid | 2-s2.0-55049142093 | - |
dc.identifier.wosid | 000260921100005 | - |
dc.identifier.bibliographicCitation | ENDOCRINE JOURNAL, v.55, no.5, pp.827 - 837 | - |
dc.relation.isPartOf | ENDOCRINE JOURNAL | - |
dc.citation.title | ENDOCRINE JOURNAL | - |
dc.citation.volume | 55 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 827 | - |
dc.citation.endPage | 837 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ENDOCRINE | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordAuthor | Leptin | - |
dc.subject.keywordAuthor | IGF-I | - |
dc.subject.keywordAuthor | PPAR | - |
dc.subject.keywordAuthor | Adipocyte | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Lipid | - |
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