Sphingosine kinase inhibitor suppresses IL-18-induced interferon-gamma production through inhibition of p38 MAPK activation in human NK cells
DC Field | Value | Language |
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dc.contributor.author | Cheon, Soyoung | - |
dc.contributor.author | Song, Seok Bean | - |
dc.contributor.author | Jung, Minkyung | - |
dc.contributor.author | Park, Yoorim | - |
dc.contributor.author | Bang, Jung-Wook | - |
dc.contributor.author | Kim, Tae Sung | - |
dc.contributor.author | Park, Hyunjeong | - |
dc.contributor.author | Kim, Cher-hyun | - |
dc.contributor.author | Yang, Yool-hee | - |
dc.contributor.author | Bang, Sa Ik | - |
dc.contributor.author | Cho, Daeho | - |
dc.date.accessioned | 2021-09-09T04:18:06Z | - |
dc.date.available | 2021-09-09T04:18:06Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-09-12 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122709 | - |
dc.description.abstract | Natural killer (NK) cells play an important role in the innate immune response. Interleukin-18 (IL-18) is a well-known interferon-gamma (IFN-gamma inducing factor, which stimulates immune response in NK and T cells. Sphingosine kinase (SPHK) catalyzes the formation of sphingosine 1-phosphate (SI P), which acts as a second messenger to function as an anti-apoptotic factor and proliferation stimulator of immune cells. In this study, to elucidate whether SPHK is involved in IL-18-induced IFN-gamma production, we measured IL-18-induced IFN-gamma production after pre-treatment with SPHK inhibitor (SKI) in NK-92MI cells. We found that IL-18-induced IFN-gamma expression was blocked by SKI pre-treatment in both mRNA and protein levels. in addition, the increased IFN-1 production by stimulation with IL-18 is mediated through both SPHK and p38 MAPK. To determine the upstream signals of SKI and p38 MAPK in IL-18-induced IFN-gamma production, phosphorylation levels of p38 MAPK was measured after SKI pre-treatment. As a result, inhibition of SPHK by SKI blocked phosphorylation of p38 MAPK, showing that SPHK activation by IL-18 is an upstream signal of p38 MAPK activation. Inhibition of SPHK by SKI also inhibited IL-18-induced IFN-gamma production in human primary NK cells. In conclusion, SPHK activation is an essential factor for IL-18-induced IFN-gamma production via p38 MAPK. (C) 2008 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | CD4 T-CELLS | - |
dc.subject | MIGRATION | - |
dc.subject | 1-PHOSPHATE | - |
dc.subject | SPHINGOSINE-1-PHOSPHATE | - |
dc.subject | TYPE-1 | - |
dc.title | Sphingosine kinase inhibitor suppresses IL-18-induced interferon-gamma production through inhibition of p38 MAPK activation in human NK cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Sung | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.06.091 | - |
dc.identifier.scopusid | 2-s2.0-47749148735 | - |
dc.identifier.wosid | 000258798700015 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.374, no.1, pp.74 - 78 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 374 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 74 | - |
dc.citation.endPage | 78 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | CD4 T-CELLS | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | 1-PHOSPHATE | - |
dc.subject.keywordPlus | SPHINGOSINE-1-PHOSPHATE | - |
dc.subject.keywordPlus | TYPE-1 | - |
dc.subject.keywordAuthor | sphingosine kinase | - |
dc.subject.keywordAuthor | interleukin-18 | - |
dc.subject.keywordAuthor | interferon-gamma | - |
dc.subject.keywordAuthor | p38MAPK | - |
dc.subject.keywordAuthor | NK cell | - |
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