Expression and secretion of the insulin-like growth factor system components by pig liver cells
DC Field | Value | Language |
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dc.contributor.author | Kim, I. | - |
dc.contributor.author | Jin, E. J. | - |
dc.contributor.author | Baik, K. | - |
dc.contributor.author | Park, C. H. | - |
dc.contributor.author | Kim, W. K. | - |
dc.contributor.author | Kang, C. W. | - |
dc.contributor.author | Ko, Y. | - |
dc.contributor.author | Jang, I. | - |
dc.contributor.author | Choi, W. S. | - |
dc.contributor.author | Lee, C. Y. | - |
dc.date.accessioned | 2021-09-09T04:34:11Z | - |
dc.date.available | 2021-09-09T04:34:11Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-09 | - |
dc.identifier.issn | 1011-2367 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122751 | - |
dc.description.abstract | The aim of the present stud), was to delineate the expression and secretion of insulin-like growth factor (IGF) system components by pig liver cells. Hepatocytes were prepared from 3-wk-old weanling piglets following a two-step collagenase perfusion procedure, after which the cells were incubated for 24 or 48 h at a density of 2x10(5) cells per 3 5-mm dish in 2-ml Williams' medium E. The cells were found to express the genes encoding IGF-I, IGF-binding proteins (IGFBPs)-2 and -3 and acid-labile subunit (ALS) by reverse transcription-polymerase chain reaction (RT-PCR) following the culture. However, IGF-I was localized to hepatocytes by immunohistochemical analysis, whereas IGFBP-3 was localized to endothelial cells, but not to hepatocytes. This indicated that the IGFBP-3 gene expression detected by RT-PCR was likely to have been contributed by unidentified non-parenchymal cells that had not been removed during the hepatocyte preparation. The conditioned culture medium (CCM) of the cells contained immunoreactive IGF-I and IGF-II, with the latter being seven-fold more abundant than the former. The CCM also contained 43-, 40- 34-, 31-kDa doublet and 26-kDa IGFBPs as examined by Western ligand blotting. The 40-, 34- and 31-kDa doublet IGFBPs were approximately three-fold as abundant as the 43- and 26-kDa IGFBPs. Moreover, the 43- and 40-kDa doublet and the 34-kDa IGFBPs were immunoprecipitable with IGFBP-3 and IGFBP-2 antibodies, respectively. Overall, these results are similar to those known in the rat, which suggests that the IGF system components are likely to be expressed and secreted in pig liver in a manner similar to that in rat liver. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC | - |
dc.subject | FACTOR-BINDING PROTEIN | - |
dc.subject | MESSENGER-RNA EXPRESSION | - |
dc.subject | ADULT-RAT SERUM | - |
dc.subject | FACTOR-I | - |
dc.subject | IGF-II | - |
dc.subject | NONPARENCHYMAL CELLS | - |
dc.subject | ENDOCRINE FACTORS | - |
dc.subject | HORMONAL-CONTROL | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | COMPLEX | - |
dc.title | Expression and secretion of the insulin-like growth factor system components by pig liver cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Y. | - |
dc.identifier.doi | 10.5713/ajas.2008.70558 | - |
dc.identifier.scopusid | 2-s2.0-56849098590 | - |
dc.identifier.wosid | 000258922100004 | - |
dc.identifier.bibliographicCitation | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, v.21, no.9, pp.1244 - 1251 | - |
dc.relation.isPartOf | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | - |
dc.citation.title | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1244 | - |
dc.citation.endPage | 1251 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001291792 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | other | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.subject.keywordPlus | FACTOR-BINDING PROTEIN | - |
dc.subject.keywordPlus | MESSENGER-RNA EXPRESSION | - |
dc.subject.keywordPlus | ADULT-RAT SERUM | - |
dc.subject.keywordPlus | FACTOR-I | - |
dc.subject.keywordPlus | IGF-II | - |
dc.subject.keywordPlus | NONPARENCHYMAL CELLS | - |
dc.subject.keywordPlus | ENDOCRINE FACTORS | - |
dc.subject.keywordPlus | HORMONAL-CONTROL | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordAuthor | IGF | - |
dc.subject.keywordAuthor | IGFBP | - |
dc.subject.keywordAuthor | gene expression | - |
dc.subject.keywordAuthor | hepatocyte | - |
dc.subject.keywordAuthor | pig | - |
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