Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Single-stranded DNA aptamers specific for antibiotics tetracyclines

Authors
Niazi, Javed H.Lee, Su JinGu, Man Bock
Issue Date
1-8월-2008
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
antibiotics; tetracyclines; aptamer; ssDNA; SELEX
Citation
BIOORGANIC & MEDICINAL CHEMISTRY, v.16, no.15, pp.7245 - 7253
Indexed
SCIE
SCOPUS
Journal Title
BIOORGANIC & MEDICINAL CHEMISTRY
Volume
16
Number
15
Start Page
7245
End Page
7253
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/122883
DOI
10.1016/j.bmc.2008.06.033
ISSN
0968-0896
Abstract
Tetracyclines (TCs) are a group of antibiotics comprising of a common tetracycline (TET) nucleus with variable X-1 and X-2 positions on 5 and 6 carbon atoms, such as oxytetracycline (OTC) and doxycycline (DOX). In this study, the tetracycline group specific (TGS) ssDNA aptamers were identified by modified SELEX method by employing tosylactivated magnetic beads (TMB) coated with OTC, TET, and DOX, respectively, as targets and counter targets. Twenty TGS-aptamers were selected, of which seven aptamers, designated as T7, T15, T19, T20, T22, T23, and T24, showed high affinity to the basic TET backbone (K-d = 63-483 nM). The specificity of these TGS-aptamers to structural analogues followed the order in which the TCs was employed during SELEX process (OTC > TET > DOX) except aptamer T22, which was highly specific to TET than OTC or DOX. Aptamers that were specific to one target molecule but fail to bind the other structurally related TCs were eliminated during counter selection steps. Three aptamers, T7, T19, and T23 contained palindromic consensus sequence motif GGTGTGG. The remaining TGS-aptamers showed many consensus sequences that are truncated forms of this palindrome forming mirror image or inverted sequences. For example, GTGG or its inverted form, GGTG motif was found in all TGS-aptamers. A consensus sequence motif TGTGCT or its truncated terminal T-residue was found in most TGS-aptamers, which is predicted to be essential for high affinity and group specificity. These TGS-aptamers have potential applications such as target drug delivery, and detection of TCs in pharmaceutical preparations and contaminated food products. (C) 2008 Elsevier Ltd. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Gu, Man Bock photo

Gu, Man Bock
융합생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE