Effects of tobacco compounds on gene expression in fetal lung fibroblasts
- Authors
- Sohn, Sung-Hwa; Kim, Ki-Nam; Kim, In Kyoung; Lee, Eun-Il; Ryu, Jae-Jun; Kim, Meyoung-Kon
- Issue Date
- 8월-2008
- Publisher
- WILEY
- Keywords
- nicotine; B(a)P; 2-Naphthylamine; tobacco compounds; gene expression profile; cDNA microarray
- Citation
- ENVIRONMENTAL TOXICOLOGY, v.23, no.4, pp.423 - 434
- Indexed
- SCIE
SCOPUS
- Journal Title
- ENVIRONMENTAL TOXICOLOGY
- Volume
- 23
- Number
- 4
- Start Page
- 423
- End Page
- 434
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122895
- DOI
- 10.1002/tox.20335
- ISSN
- 1520-4081
- Abstract
- The goal of this study was to determine the effects of tobacco compounds on gene expression in a human fetal lung cell line (W138). In the present study, we investigated the effects of tobacco compounds (nicotine, benzo(a)pyrene (B(a)P), and 2-Naphthylamine) on gene expression profiles in human fetal fibroblasts using cDNA microarray and real-time PCR. W138 cells were cultured in Eagle's minimum essential medium (MEM) supplemented with 10% fetal bovine serum, 2% 200 mM L-glutamine, and a 2% penicillin and streptomycin solution. Tissue culture flasks (T-25 cm(2)) containing confluent lung fibroblasts were incubated at 37 degrees C for 24 h with 5 mL of medium supplemented with 10 mu M of a tobacco compound (nicotine, B(a)P, or 2-Naphthylamine). The gene expression profiles for the W138 cells varied depending on the tobacco compound. The cDNA microarray analysis revealed that apoptosis-related genes such as DNASE2, MADD, MST1, NME3, RARG, TNFRSF1A, BAD, and DFFB genes were down-regulated in tobacco compound-treated W138 cells. We also observed significant increases in Arnt gene expression by real-time PCR in tobacco compound-treated W138 cells. Tobacco compounds can affect apoptosis, immunity, and growth in W138 cells. A microarray-based genomic survey is a high-throughput approach for the evaluation of gene expression in cell lines treated with tobacco compounds. (C) 2008 Wiley Periodicals, Inc.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.