Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila
DC Field | Value | Language |
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dc.contributor.author | Lim, Do Hwan | - |
dc.contributor.author | Kim, Jung | - |
dc.contributor.author | Kim, Sanguk | - |
dc.contributor.author | Carthew, Richard W. | - |
dc.contributor.author | Lee, Young Sik | - |
dc.date.accessioned | 2021-09-09T06:00:01Z | - |
dc.date.available | 2021-09-09T06:00:01Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-07-04 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123034 | - |
dc.description.abstract | The Drosophila RNase III enzyme Dicer-2 processes double-stranded RNA (dsRNA) precursors into small interfering RNAs (siRNAs). It also interacts with the siRNA product and R2D2 protein to facilitate the assembly of an RNA-induced silencing complex (RISC) that mediates RNA interference. Here, we characterized six independent missense mutations in the dicer-2 gene. Four mutations (P8S, L188F, R269W, and P365L) in the DExH helicase domain reduced dsRNA processing activity. Two mutations were located within an RNase III domain. P1496L caused a loss of dsRNA processing activity comparable to a null dicer-2 mutation. A1453T strongly reduced both dsRNA processing and RISC activity, and decreased the levels of Dicer-2 and R2D2 proteins, suggesting that this mutation destabilizes Dicer-2. We also found that the carboxyl-terminal region of R2D2 is essential for Dicer-2 binding. These results provide further insight into the structure-function relationship of Dicer, which plays a critical role in the siRNA pathway. (C) 2008 Elsevier Inc. All rights reserved | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | DOUBLE-STRANDED-RNA | - |
dc.subject | INTERFERENCE | - |
dc.subject | COMPLEXES | - |
dc.subject | MECHANISM | - |
dc.subject | CLEAVAGE | - |
dc.subject | R2D2 | - |
dc.title | Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Young Sik | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.04.118 | - |
dc.identifier.scopusid | 2-s2.0-43549116789 | - |
dc.identifier.wosid | 000256319100036 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.371, no.3, pp.525 - 530 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 371 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 525 | - |
dc.citation.endPage | 530 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | R2D2 | - |
dc.subject.keywordAuthor | RNA interference | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Drosophila | - |
dc.subject.keywordAuthor | mutation | - |
dc.subject.keywordAuthor | dicer-2 | - |
dc.subject.keywordAuthor | R2D2 | - |
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