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Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection

Authors
Kim, JBaek, JKim, HLee, KLee, S
Issue Date
31-3월-2006
Publisher
ELSEVIER SCIENCE SA
Keywords
carbon nanotube; PDMS; glucose-detection; microfluidics
Citation
SENSORS AND ACTUATORS A-PHYSICAL, v.128, no.1, pp.7 - 13
Indexed
SCIE
SCOPUS
Journal Title
SENSORS AND ACTUATORS A-PHYSICAL
Volume
128
Number
1
Start Page
7
End Page
13
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123148
DOI
10.1016/j.sna.2005.12.039
ISSN
0924-4247
Abstract
We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-mu m-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-mu m-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations. (c) 2006 Elsevier B.V. All rights reserved.
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