Hyaluronic acid-polyethyleneimine conjugate for target specific intracellular delivery of siRNA
DC Field | Value | Language |
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dc.contributor.author | Jiang, Ge | - |
dc.contributor.author | Park, Kitae | - |
dc.contributor.author | Kim, Jiseok | - |
dc.contributor.author | Kim, Ki Su | - |
dc.contributor.author | Oh, Eun Ju | - |
dc.contributor.author | Kang, Hyungu | - |
dc.contributor.author | Han, Su-Eun | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.contributor.author | Park, Tae Gwan | - |
dc.contributor.author | Hahn, Sei Kwang | - |
dc.date.accessioned | 2021-09-09T07:02:21Z | - |
dc.date.available | 2021-09-09T07:02:21Z | - |
dc.date.issued | 2008-07 | - |
dc.identifier.issn | 0006-3525 | - |
dc.identifier.issn | 1097-0282 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123294 | - |
dc.description.abstract | A novel target specific small interfering RNA (siRNA) delivery system was successfully developed using I polyethyleneimine (PEI)-hyaluronic acid (HA) conjugate. Anti-PGL3-Luc siRNA was used as a model system suppressing the PGL3-Luc gene expression. The siRNA/PEI-HA complex with an average size of ca.21 nm appeared to be formed by electrostatic interaction between the negatively charged siRNA and the positively charged PEI of PEI-HA conjugate. The cytotoxicity of siRNA/PEI-HA complex to B16F1 cells was lower than that of siRNA/PEI complex according to the MTTassay. When B16F1 and HEK-293 cells were treated with fluorescein isothiocyanate (FITC) labeled siRNA/PEI-HA complex, B16F1 cells, with a lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), showed higher green fluorescent intensity than HEK-293 cells because of the HA receptor mediated endocytosis of the complex. Accordingly, the PGL3-Luc gene silencing of anti-PGL3-Luc siRNA/PEI-HA complex was more efficient in B16F1 cells than in HEK-293 cells. In addition, the inhibited PGL3-Luc gene silencing effect in the presence of free HA in the transfection medium revealed that siRNA/HA-PEI complex was selectively taken up to B16F1 cells via HA receptor mediated endocytosis. All these results demonstrated that the intracellular delivery of anti-PGL3-Luc siRNA/PEI-HA complex could be facilitated by the HA receptor mediated endocytosis. (c) 2008 Wiley Periodicals, Inc. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Hyaluronic acid-polyethyleneimine conjugate for target specific intracellular delivery of siRNA | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/bip.20978 | - |
dc.identifier.scopusid | 2-s2.0-49149112031 | - |
dc.identifier.wosid | 000255313700009 | - |
dc.identifier.bibliographicCitation | BIOPOLYMERS, v.89, no.7, pp 635 - 642 | - |
dc.citation.title | BIOPOLYMERS | - |
dc.citation.volume | 89 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 635 | - |
dc.citation.endPage | 642 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | POLYELECTROLYTE COMPLEX MICELLES | - |
dc.subject.keywordPlus | SUSTAINED-RELEASE FORMULATION | - |
dc.subject.keywordPlus | MEDIATED GENE DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | RNA INTERFERENCE | - |
dc.subject.keywordPlus | SODIUM HYALURONATE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordAuthor | hyaluronic acid | - |
dc.subject.keywordAuthor | polyethyleneimine | - |
dc.subject.keywordAuthor | small interfering RNA | - |
dc.subject.keywordAuthor | LYVE-1 HA receptor | - |
dc.subject.keywordAuthor | intracellular gene delivery | - |
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