Maltose binding protein facilitates high-level expression and functional purification of the chemokines RANTES and SDF-1 alpha from Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | Cho, Hee-Jeong | - |
dc.contributor.author | Lee, Young | - |
dc.contributor.author | Chang, Rae Sung | - |
dc.contributor.author | Hahm, Moon-Sun | - |
dc.contributor.author | Kim, Myung-Kuk | - |
dc.contributor.author | Kim, Young Bong | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2021-09-09T07:12:52Z | - |
dc.date.available | 2021-09-09T07:12:52Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-07 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123328 | - |
dc.description.abstract | The chemokines RANTES (regulated on activation, normal T cell expressed and secreted) and SDF-1 alpha (stromal cell-derived factor-la) are important regulators of leukocyte trafficking and homing. Chemokines form insoluble inclusion bodies when expressed in Escherichia coli (E. coli), resulting in low yields of soluble protein. We have developed a novel chemokine expression system that generates a high amount of soluble protein and uses a simple purification scheme. We cloned different types of RANTES and SDF-1 alpha fused to either maltose binding protein (MBP) or glutathione-S-transferase (GST) and expressed the fusion proteins in E. coli under various conditions. We found that the yield of soluble chemokine is influenced by the type of fusion partner. Fusion to MBP resulted in a higher yield of total and soluble chemokine compared to GST. Under optimized conditions, the yield of soluble MBP-RANTES and MBP-SDF-1 alpha was 2.5- and 4.5-fold higher than that of the corresponding GST-fusion protein, respectively. Recombinant chemokine fusion proteins exhibited specific binding activity to chemokine receptors. These results demonstrate that the use of MBP-fusion proteins may provide an approach to generating high yields of soluble and functional chemokines, such as RANTES and SDF-1 alpha. (c) 2008 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | FUSION PROTEINS | - |
dc.subject | ENHANCED IMMUNOGENICITY | - |
dc.subject | AFFINITY | - |
dc.subject | MECHANISMS | - |
dc.subject | STRATEGIES | - |
dc.subject | DISEASE | - |
dc.subject | TARGETS | - |
dc.subject | SURFACE | - |
dc.subject | CXCR4 | - |
dc.subject | ALPHA | - |
dc.title | Maltose binding protein facilitates high-level expression and functional purification of the chemokines RANTES and SDF-1 alpha from Escherichia coli | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Yu-Kyoung | - |
dc.identifier.doi | 10.1016/j.pep.2008.03.018 | - |
dc.identifier.scopusid | 2-s2.0-43649096700 | - |
dc.identifier.wosid | 000257231400007 | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, v.60, no.1, pp.37 - 45 | - |
dc.relation.isPartOf | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.title | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.volume | 60 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 37 | - |
dc.citation.endPage | 45 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | FUSION PROTEINS | - |
dc.subject.keywordPlus | ENHANCED IMMUNOGENICITY | - |
dc.subject.keywordPlus | AFFINITY | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | TARGETS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | CXCR4 | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordAuthor | fusion protein | - |
dc.subject.keywordAuthor | RANTES | - |
dc.subject.keywordAuthor | SDF-1 alpha | - |
dc.subject.keywordAuthor | chemokine | - |
dc.subject.keywordAuthor | Escherichia coli | - |
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