Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Identification of a novel human Rad51 variant that promotes DNA strand exchange

Authors
Park, Jung-YoungYoo, Han-WookKim, Bok-RyangPark, RaekilChoi, Sang-YunKim, Youngho
Issue Date
6월-2008
Publisher
OXFORD UNIV PRESS
Citation
NUCLEIC ACIDS RESEARCH, v.36, no.10, pp.3226 - 3234
Indexed
SCIE
SCOPUS
Journal Title
NUCLEIC ACIDS RESEARCH
Volume
36
Number
10
Start Page
3226
End Page
3234
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123452
DOI
10.1093/nar/gkn171
ISSN
0305-1048
Abstract
Rad51 plays a key role in the repair of DNA doublestrand breaks through homologous recombination, which is the central process in the maintenance of genomic integrity. Five paralogs of the human Rad51 gene (hRad51) have been identified to date, including hRad51B, hRad51C, hRad51D, Xrcc2 and Xrcc3. In searches of additional hRad51 paralogs, we identified a novel hRad51 variant that lacked the sequence corresponding to exon 9 (hRad51-Dex9). The expected amino acid sequence of hRad51-Dex9 showed a frame- shift at codon 259, which resulted in a truncated C-terminus. RT-PCR analysis revealed that both hRad51 and hRad51-Dex9 were prominently expressed in the testis, but that there were subtle differences in tissue specificity. The hRad51- Delta ex9 protein was detected as a 31-kDa protein in the testis and localized at the nucleus. In addition, the hRad51-Delta ex9 protein showed a DNA-strand exchange activity comparable to that of hRad51. Taken together, these results indicate that hRad51-Delta ex9 promotes homologous pairing and DNA strand exchange in the nucleus, suggesting that alternative pathways in hRad51- or hRad51-Delta ex9-dependent manners exist for DNA recombination and repair.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE