Integrated catalytic activity of patterned multilayer films based on pH-induced electrostatic properties of enzymes

Abstract

Patterned multilayer structures are fabricated on enzymatic surfaces using pH-induced variations in the electrostatic properties of these surfaces, as schematically illustrated in the figure. Notably, this process does not require photolithography or the use of hydrophobic components that could lead to a loss of enzymatic activity. Highly effective electrochemical sensors incorporating metal nanoparticles and biocatalysts are fabricated using this approach.