5-azacytidine induces cardiac differentiation of P19 embryonic stem cells
DC Field | Value | Language |
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dc.contributor.author | Choi, SC | - |
dc.contributor.author | Yoon, J | - |
dc.contributor.author | Shim, WJ | - |
dc.contributor.author | Ro, YM | - |
dc.contributor.author | Lim, DS | - |
dc.date.accessioned | 2021-09-09T08:40:40Z | - |
dc.date.available | 2021-09-09T08:40:40Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2004-12-31 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123585 | - |
dc.description.abstract | The P19 embryonal carcinoma cell line is a useful model cells for studies on cardiac differentiation. However, its low efficacy of differentiation hampers its usefulness. We investigated the effect of 5-azacytidine (5-aza) on P19 cells to differentiate into a high-efficacy cardiomyocytes. Embryoid-body-like structures were formed after 6 days with 1 muM of 5-aza in a P19 cell monolayer culture, beating cell clusters first observed on day 12, and, the production of beating cell clusters increased by 80.1% (29 of 36-wells) after 18 days. In comparison, the spontaneous beating cells was 33.3% (12 of 36-wells) for the untreated control cells. In response to 1 muM of 5-aza, P19 cells expressed bone morphogenetic protein-2 (BMP-2), BMP-4, Bmpr1a and Smad1 at day 6 or 9, and also cardiac markers such as GATA-4, Nkx2.5, cardiac troponin 1, and desmin were up-regulated in a time-dependent manner after induction of BMP signaling molecules. Immunocytochemistry revealed the expression of smooth muscle alpha-actin, sarcomeric alpha- actinin, cardiac myosin heavy chain, cardiac troponin T and desmin, respectively. The proportion of sarcomeric alpha- actinin positive cells accounted for 6.48% on day 15 after 5-aza exposure as measured by flow cytometry. This study has demonstrated that 5-aza induces differentiation of P19 cells into cardiomyocytes in a confluent monolayer culture in the absence of prior embryoid formation and dimethyl sulfoxide exposure, depending in part on alteration of BMP signaling molecules. These results suggest that 5-aza treatment could be used as a new method for cardiac differentiation in P19 cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | DNA METHYLATION | - |
dc.subject | CARCINOMA-CELLS | - |
dc.subject | CYTIDINE ANALOGS | - |
dc.subject | PROGENITOR CELLS | - |
dc.subject | CARDIOMYOCYTES | - |
dc.subject | EXPRESSION | - |
dc.subject | ADULT | - |
dc.subject | LINE | - |
dc.subject | 5-AZA-2&apos | - |
dc.subject | -DEOXYCYTIDINE | - |
dc.subject | CARDIOMYOGENESIS | - |
dc.title | 5-azacytidine induces cardiac differentiation of P19 embryonic stem cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, SC | - |
dc.identifier.doi | 10.1038/emm.2004.66 | - |
dc.identifier.scopusid | 2-s2.0-12344266614 | - |
dc.identifier.wosid | 000226464100003 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.36, no.6, pp.515 - 523 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 36 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 515 | - |
dc.citation.endPage | 523 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001100264 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | DNA METHYLATION | - |
dc.subject.keywordPlus | CARCINOMA-CELLS | - |
dc.subject.keywordPlus | CYTIDINE ANALOGS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | CARDIOMYOCYTES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ADULT | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordPlus | 5-AZA-2&apos | - |
dc.subject.keywordPlus | -DEOXYCYTIDINE | - |
dc.subject.keywordPlus | CARDIOMYOGENESIS | - |
dc.subject.keywordAuthor | aza compounds | - |
dc.subject.keywordAuthor | bone morphogenetic | - |
dc.subject.keywordAuthor | proteins | - |
dc.subject.keywordAuthor | cardiac myocytes | - |
dc.subject.keywordAuthor | cell differentiation | - |
dc.subject.keywordAuthor | gene expression | - |
dc.subject.keywordAuthor | stem cells | - |
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