Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, MK | - |
dc.contributor.author | Oh, S | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Im, H | - |
dc.contributor.author | Ryu, YM | - |
dc.contributor.author | Oh, E | - |
dc.contributor.author | Lee, J | - |
dc.contributor.author | Lee, E | - |
dc.contributor.author | Sul, D | - |
dc.date.accessioned | 2021-09-09T08:42:14Z | - |
dc.date.available | 2021-09-09T08:42:14Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2004-10-31 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123593 | - |
dc.description.abstract | In this study, we investigated the effects of PAHs and dioxin on mRNA and plasma protein expression using genomic and proteomic analysis for automobile emission inspectors and waste incineration workers. About 54 workers from automobile emission inspection offices, 31 workers from waste incinerating company and 84 unexposed healthy subjects were enrolled in this study. Urine and air samples were collected and analyzed by HPLC and GCIMS. Comet assays were carried out to evaluate any DNA damage in mononuclear and polyinuclear cells. A significant difference in Olive tail moments in mononuclear cells was observed between exposed and control subjects (P < 0.0001). To examine the differences of the gene expression profile in automobile emission inspectors and waste incineration workers, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 total genes. The gene expression profiles showed that 11 genes were up-regulated and 4 genes were down-regulated in waste incinerating workers as compared with controls. Plasma proteins were analyzed by 2-dimentional electrophoresis with pH 3-10 NL IPG Dry strip. The protein expression profiles showed that 8 proteins were upregulated and 1 protein, haptoglobin, was downregulated in automobile emission inspectors and waste incineration workers. Serum paraoxonase/ arylesterase was found only in the plasma of waste incineration workers. The expression of genes and proteins involved in oxidative stress were up-regulated in both automobile emission inspectors and waste incineration workers. Several proteins, such as transthyrethin, sarcolectin and haptoglobin, that were highly up- or down-regulated, could serve as biological monitoring markers for future study. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | HUMAN-URINE | - |
dc.subject | EXPRESSION | - |
dc.subject | DIOXINS | - |
dc.subject | 1-HYDROXYPYRENE | - |
dc.subject | 2-NAPHTHOL | - |
dc.subject | BIOMARKERS | - |
dc.subject | CANCER | - |
dc.subject | HSP70 | - |
dc.subject | CONTAMINATION | - |
dc.title | Evaluation of biological monitoring markers using genomic and proteomic analysis for automobile emission inspectors and waste incinerating workers exposed to polycyclic aromatic hydrocarbons or 2,3,7,8,-tetracholrodedibenzo-p-dioxins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sul, D | - |
dc.identifier.doi | 10.1038/emm.2004.52 | - |
dc.identifier.scopusid | 2-s2.0-9344263443 | - |
dc.identifier.wosid | 000225359200002 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.36, no.5, pp.396 - 410 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 36 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 396 | - |
dc.citation.endPage | 410 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | HUMAN-URINE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DIOXINS | - |
dc.subject.keywordPlus | 1-HYDROXYPYRENE | - |
dc.subject.keywordPlus | 2-NAPHTHOL | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | HSP70 | - |
dc.subject.keywordPlus | CONTAMINATION | - |
dc.subject.keywordAuthor | biological monitoring markers | - |
dc.subject.keywordAuthor | dioxins | - |
dc.subject.keywordAuthor | genomic | - |
dc.subject.keywordAuthor | polycyclic aromatic hydrocarbons | - |
dc.subject.keywordAuthor | proteomic | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.