Effects of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on the expression of inflammatory cytokines and apoptosis induction in rheumatoid synovial fibroblasts and monocytes
DC Field | Value | Language |
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dc.contributor.author | Ji, JD | - |
dc.contributor.author | Cheon, H | - |
dc.contributor.author | Jun, JB | - |
dc.contributor.author | Choi, SJ | - |
dc.contributor.author | Kim, YR | - |
dc.contributor.author | Lee, YH | - |
dc.contributor.author | Kim, TH | - |
dc.contributor.author | Chae, IJ | - |
dc.contributor.author | Song, GG | - |
dc.contributor.author | Yoo, DH | - |
dc.contributor.author | Kim, SY | - |
dc.contributor.author | Sohn, J | - |
dc.date.accessioned | 2021-09-09T08:48:42Z | - |
dc.date.available | 2021-09-09T08:48:42Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2001-11 | - |
dc.identifier.issn | 0896-8411 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123628 | - |
dc.description.abstract | This study was performed to investigate whether peroxisome proliterator-activated receptor-gamma (PPAR-gamma) exerted an anti-inflammatory effect on rheumatoid synovial cells and inhibited dysregulated proliferation. The expression of PPAR-gamma mRNA in cultured human synoviocytes and THP-1 cells was analysed by RT-PCR. PPAR-gamma was expressed in normal, osteoarthritis (OA), rheumatoid arthritis (RA) synovial cells as well as a human monocytic cell line, THP-1. In RA and OA synoviocytes, the induction of inflammatory cytokine mRNA expression such as TNF-a and IL-1 beta was significantly inhibited by the natural PPAR-gamma agonist 15 deoxy-Delta (12,14) prostaglandin J(2), (15d-PGJ,). The effect of PPAR-y on the nuclear factor (NF)-KB activity was tested by electrophoretic mobility shift assay (EMSA). Both troglitazone and 15d-PGJ(2) markedly inhibited TNF-a-induced NF-KB activation at 30 muM. However, PPAR-y agonist neither reduced proliferation nor induced apoptosis in RA synoviocytes when measured by XTT assay and fluorescence activated cell sorter (FACS) analysis. In contrast, it induced apoptosis in a dose-dependent manner in THP-1 cells and augmented INF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis as well. In conclusion, these data demonstrate that PPAR-gamma is expressed in human synoviocytes and THP-1 cells, and the PPAR-7 activation inhibits expression of inflammatory cytokines in RA synoviocytes. Furthermore, PPAR-y activation induces apoptosis by itself and augments TRAIL/ Apo2L-induced apoptosis in THP-1 cells. These results suggest that PPAR-7 agonists may provide a new therapeutic approach for RA. (C) 2001 Academic Press. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS LTD | - |
dc.subject | BREAST-CANCER CELLS | - |
dc.subject | 15-DEOXY-DELTA(12,14)-PROSTAGLANDIN J(2) | - |
dc.subject | KAPPA-B | - |
dc.subject | ADIPOCYTE DIFFERENTIATION | - |
dc.subject | IN-VITRO | - |
dc.subject | LIGAND | - |
dc.subject | MACROPHAGES | - |
dc.subject | GROWTH | - |
dc.subject | ALPHA | - |
dc.subject | TRAIL | - |
dc.title | Effects of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on the expression of inflammatory cytokines and apoptosis induction in rheumatoid synovial fibroblasts and monocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sohn, J | - |
dc.identifier.doi | 10.1006/jaut.2001.0542 | - |
dc.identifier.scopusid | 2-s2.0-0035508145 | - |
dc.identifier.wosid | 000172427400006 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AUTOIMMUNITY, v.17, no.3, pp.215 - 221 | - |
dc.relation.isPartOf | JOURNAL OF AUTOIMMUNITY | - |
dc.citation.title | JOURNAL OF AUTOIMMUNITY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 215 | - |
dc.citation.endPage | 221 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | 15-DEOXY-DELTA(12,14)-PROSTAGLANDIN J(2) | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | LIGAND | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | TRAIL | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Inflammatory cytokine | - |
dc.subject.keywordAuthor | NF-κB | - |
dc.subject.keywordAuthor | PPAR-γ | - |
dc.subject.keywordAuthor | Rheumatoid arthritis | - |
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