Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells
- Authors
- Choi, Seung-Cheol; Shim, Wan-Joo; Lim, Do-Sun
- Issue Date
- 5월-2008
- Publisher
- SPRINGER
- Keywords
- cardiomyogenic differentiation; dual promoters; endothelial differentiation; mesenchymal stem cells; reporter genes
- Citation
- BIOTECHNOLOGY LETTERS, v.30, no.5, pp.835 - 843
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 30
- Number
- 5
- Start Page
- 835
- End Page
- 843
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/123670
- DOI
- 10.1007/s10529-007-9631-z
- ISSN
- 0141-5492
- Abstract
- Vectors encoding reporter genes driven by cardiac specific myosin light chain- 2v ( MLC- 2v), endothelial cell- specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation- monitoring vector ( pMLC- 2v- DsRed), endothelial cell- specific monitoring vectors ( pFlk1- EGFP, pTie2- EGFP) as well as the dual promoter driven- reporter genes ( pMLC- 2v- DsRed- Flk1- EGFP, pMLC- 2v- DsRed-Tie2- EGFP) were specifically expressed in the Sca-1(+) bone marrow mesenchymal stem cells ( BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell- specific promoter- driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.
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- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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