Novel cationic cholesterol derivative-based liposomes for serum-enhanced delivery of siRNA
DC Field | Value | Language |
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dc.contributor.author | Han, Su-Eun | - |
dc.contributor.author | Kang, Hyungu | - |
dc.contributor.author | Shim, Ga Yong | - |
dc.contributor.author | Suh, Min Sung | - |
dc.contributor.author | Kim, Sun Jae | - |
dc.contributor.author | Kim, Jin-Seok | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2021-09-09T09:29:11Z | - |
dc.date.available | 2021-09-09T09:29:11Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-04-02 | - |
dc.identifier.issn | 0378-5173 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123746 | - |
dc.description.abstract | Most cationic liposomes used for gene delivery suffer from reduced transfection efficiency in the presence of serum. In this study, we report serum-enhanced delivery efficiency of siRNA via the use of newly synthesized liposomes that contain cationic lipids. Two cholesterol derivatives, cholesteryloxypropan-1-amine (COPA) and cholesteryl-2-an-noethylcarbamate (CAEC), were synthesized. A fluorescein label was then used to visualize cellular uptake of small interfering RNA (siRNA) via COPA or CAEC-based liposomes. The presence of serum had different effects on the cellular delivery of siRNA when siRNA was complexed to different cationic liposomes. CAEC-based liposomes showed significantly reduced cellular delivery of siRNA in serum-containing media as compared to serum-free media. Conversely, COPA-based liposomes (COPA-L) provided serum-enhanced delivery of siRNA in Hepal-6, A549, and Hela cell lines. Following delivery of the oncogene survivin-specific siRNA, COPA-L reduced the mRNA expression levels of the target gene more efficiently than did Lipofectamine 2000. The delivery of green fluorescent protein-specific siRNA with COPA-L reduced the expression of green fluorescent protein in 293T stable cell lines. The apoptosis of Hepal-6 significantly increased by delivery of survivin-specific siRNA by COPA-L. Additionally, Hepal-6, A549, and Hela cells were > 80% viable after treatment with COPA-L. These results suggest that the newly synthesized cholesterol derivative, COPA-L, could be further developed as a serum-enhanced delivery system of siRNA. (c) 2007 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | TRANSFECTION EFFICIENCY | - |
dc.subject | LIPOFECTION EFFICIENCY | - |
dc.subject | PLASMID DNA | - |
dc.subject | GENE | - |
dc.subject | LIPOPLEXES | - |
dc.subject | COMPLEXES | - |
dc.subject | DOPE | - |
dc.subject | SIZE | - |
dc.title | Novel cationic cholesterol derivative-based liposomes for serum-enhanced delivery of siRNA | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Yu-Kyoung | - |
dc.identifier.doi | 10.1016/j.ijpharm.2007.11.026 | - |
dc.identifier.scopusid | 2-s2.0-40149098328 | - |
dc.identifier.wosid | 000255320000030 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.353, no.1-2, pp.260 - 269 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF PHARMACEUTICS | - |
dc.citation.title | INTERNATIONAL JOURNAL OF PHARMACEUTICS | - |
dc.citation.volume | 353 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 260 | - |
dc.citation.endPage | 269 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | LIPOFECTION EFFICIENCY | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | LIPOPLEXES | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | DOPE | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordAuthor | cationic lipids | - |
dc.subject.keywordAuthor | cholesterol derivatives | - |
dc.subject.keywordAuthor | serum stability | - |
dc.subject.keywordAuthor | siRNA delivery | - |
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