Rapid functional identification of putative genes based on the combined in vitro protein synthesis with mass spectrometry: A tool for functional genomics
DC Field | Value | Language |
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dc.contributor.author | Kim, June-Hyung | - |
dc.contributor.author | Jang, Kyoung-Soon | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Lee, Ji-Hye | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.date.accessioned | 2021-09-09T09:29:23Z | - |
dc.date.available | 2021-09-09T09:29:23Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-04-01 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123747 | - |
dc.description.abstract | For the rapid identification of functional activity of unknown genes from a sequence database, a new method based on in vitro protein synthesis combined with mass spectrometry was developed. To discriminate their subtle enzymatic activity, in vitro synthesized and one-step purified lipolytic enzymes, such as lipA and lipB from Bacillus subtilis and an unknown protein ybfF from Escherichia coli, were reacted with a mixture of triglycerides with different carbon chain lengths. Using direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis of reaction product, all three enzymes were revealed to have strong esterase activity rather than true lipase activity, which has no reactivity on long-chain fatty acids such as triolein. These results were also confirmed by classical color assay using p-nitrophenyl butyrate (pNPB) and p-nitrophenyl palmitate (pNPP) as representative lipolytic substrates. (c) 2008 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | STRUCTURAL GENOMICS | - |
dc.title | Rapid functional identification of putative genes based on the combined in vitro protein synthesis with mass spectrometry: A tool for functional genomics | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.ab.2008.01.007 | - |
dc.identifier.scopusid | 2-s2.0-39749119783 | - |
dc.identifier.wosid | 000254060100002 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.375, no.1, pp.11 - 17 | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 375 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 11 | - |
dc.citation.endPage | 17 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | STRUCTURAL GENOMICS | - |
dc.subject.keywordAuthor | enzyme activity | - |
dc.subject.keywordAuthor | esterase | - |
dc.subject.keywordAuthor | in vitro protein synthesis | - |
dc.subject.keywordAuthor | lipase | - |
dc.subject.keywordAuthor | MALDI-MS | - |
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