Impaired ribosome biogenesis disrupts the integration between morphogenesis and nuclear duplication during the germination of Aspergillus fumigatus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bhabhra, Ruchi | - |
dc.contributor.author | Richie, Daryl L. | - |
dc.contributor.author | Kim, H. Stanley | - |
dc.contributor.author | Nierman, William C. | - |
dc.contributor.author | Fortwendel, Jarrod | - |
dc.contributor.author | Aris, John P. | - |
dc.contributor.author | Rhodes, Judith C. | - |
dc.contributor.author | Askew, David S. | - |
dc.date.accessioned | 2021-09-09T09:36:22Z | - |
dc.date.available | 2021-09-09T09:36:22Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-04 | - |
dc.identifier.issn | 1535-9778 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123783 | - |
dc.description.abstract | Aspergillus fumigatus is an important opportunistic fungal pathogen that is responsible for high mortality rates in the immunosuppressed population. CgrA, the A. fumigatus ortholog of a Saccharomyces cerevisiae nucleolar protein involved in ribosome biogenesis, contributes to the virulence of this fungus by supporting rapid growth at 37 degrees C. To determine how CgrA affects ribosome biogenesis in A. fumigatus, polysome profile and ribosomal subunit analyses were performed on both wild-type A. fumigatus and a Delta cgrA mutant. The loss of CgrA was associated with a reduction in the level of 80S monosomes as well as an imbalance in the 60S:40S subunit ratio and the appearance of half-mer ribosomes. The gene expression profile in the Delta cgrA mutant revealed increased abundance of a subset of translational machinery mRNAs relative to the wild type, suggesting a potential compensatory response to CgrA deficiency. Although Delta cgrA conidia germinated normally at 22 degrees C, they swelled excessively when incubated at 37 degrees C and accumulated abnormally high numbers of nuclei. This hypernucleated phenotype could be replicated pharmacologically by germinating wild-type conidia under conditions of reductive stress. These findings indicate that the germination process is particularly vulnerable to global disruption of protein synthesis and suggest that CgrA is involved in both ribosome biogenesis and polarized cell growth in A. fumigatus. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | DEPENDENT RNA HELICASE | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | FUNGAL-INFECTIONS | - |
dc.subject | PROTEIN | - |
dc.subject | YEAST | - |
dc.subject | NIDULANS | - |
dc.subject | POLARITY | - |
dc.subject | IDENTIFICATION | - |
dc.subject | EPIDEMIOLOGY | - |
dc.subject | MYCELIUM | - |
dc.title | Impaired ribosome biogenesis disrupts the integration between morphogenesis and nuclear duplication during the germination of Aspergillus fumigatus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, H. Stanley | - |
dc.identifier.doi | 10.1128/EC.00412-07 | - |
dc.identifier.scopusid | 2-s2.0-42249112431 | - |
dc.identifier.wosid | 000258666100004 | - |
dc.identifier.bibliographicCitation | EUKARYOTIC CELL, v.7, no.4, pp.575 - 583 | - |
dc.relation.isPartOf | EUKARYOTIC CELL | - |
dc.citation.title | EUKARYOTIC CELL | - |
dc.citation.volume | 7 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 575 | - |
dc.citation.endPage | 583 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Mycology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Mycology | - |
dc.subject.keywordPlus | DEPENDENT RNA HELICASE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | FUNGAL-INFECTIONS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | NIDULANS | - |
dc.subject.keywordPlus | POLARITY | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | EPIDEMIOLOGY | - |
dc.subject.keywordPlus | MYCELIUM | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.