Screening of lactobacilli derived from chicken feces and partial characterization of Lactobacillus acidophilus A12 as animal probiotics
- Authors
- Lee, Na-Kyoung; Yun, Cheol-Won; Kim, Seung Wook; Chang, Hyo-Hl; Kang, Chang-Won; Paik, Hyun-Dong
- Issue Date
- 2월-2008
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Lactobacillus acidophilus; chicken feces; animal probiotics; interleukin-1 alpha
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.2, pp.338 - 342
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 2
- Start Page
- 338
- End Page
- 342
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/124174
- ISSN
- 1017-7825
- Abstract
- This study was performed to screen and select Lactobacillus strains from chicken feces for probiotic use in animals. Of these strains, strain A12 had the highest immunostimulatory effect. Therefore, strain A 12 was characterized as a potential probiotic. Strain A12 was tentatively identified as Lactobacillus acidophilus A12, using the API 50 CHL kit based on a 99.9% homology. L. acidophilus A12 was highly resistant to artificial gastric juice (pH 2.5) and bile acid (oxgall). Based on results from the API ZYM kit, leucine arylamidase, crystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, alpha-galactosidase, beta-galactosidase, beta-glucosidase, P-glucosidase, and N-acetyl-beta-glucosamidase were produced by strain A12. L. acidophilus A12 showed resistance to several antibiotics (nisin, gentamicin, and erythromycin). The amount of interleukin (IL)-1 alpha in 20x concentrated supernatant from L. acidophilus A12 was approximately 156 pg/ml. With regard to antioxidant activity, L. acidophilus A12 supernatant showed 60.6% DPPH radical scavenging activity. These results demonstrate the potential use of L. acidophilus A12 as health-promoting probiotics.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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