PIAS1 enhances SUMO-1 modification and the transactivation activity of the major immediate-early IE2 protein of human cytomegalovirus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, JM | - |
dc.contributor.author | Kang, HJ | - |
dc.contributor.author | Lee, HR | - |
dc.contributor.author | Choi, CY | - |
dc.contributor.author | Jang, WJ | - |
dc.contributor.author | Ahn, JH | - |
dc.date.accessioned | 2021-09-09T12:24:48Z | - |
dc.date.available | 2021-09-09T12:24:48Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2003-12-04 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/124346 | - |
dc.description.abstract | The protein inhibitor of activated STAT1 (PIAS1), known to be a small ubiquitin-like modifier (SUMO) E3 ligase, was found to interact with the human cytomegalovirus IE2 protein. We found that the sumoylation of IE2 was markedly enhanced by wild-type PIAS1 but not by a mutant containing a Cys to Ser substitution at position 351 (C351S) within the RING finger-like domain. In target reporter gene assays, wild-type PIAS1, but not the C351S mutant, enhanced the IE2-mediated transactivations of viral polymerase promoter and cellular cyclin E promoter and this augmentation required the intact sumoylation sites of IE2. Our results suggest that PIAS1 acts as a SUMO E3 ligase toward IE2 and that it may regulate the transactivation function of IE2. To our knowledge, IE2 is the first viral target found to be regulated by a SUMO E3 ligase. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | REGULATORY PROTEIN | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | CYCLIN-E | - |
dc.subject | COVALENT MODIFICATION | - |
dc.subject | ANDROGEN RECEPTOR | - |
dc.subject | DNA-REPLICATION | - |
dc.subject | NUCLEAR-BODIES/ | - |
dc.subject | E3 LIGASE | - |
dc.subject | ACTIVATION | - |
dc.subject | CONJUGATION | - |
dc.title | PIAS1 enhances SUMO-1 modification and the transactivation activity of the major immediate-early IE2 protein of human cytomegalovirus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, HR | - |
dc.identifier.doi | 10.1016/S0014-5793(03)01268-7 | - |
dc.identifier.scopusid | 2-s2.0-0344872513 | - |
dc.identifier.wosid | 000187121400026 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.555, no.2, pp.322 - 328 | - |
dc.relation.isPartOf | FEBS LETTERS | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 555 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 322 | - |
dc.citation.endPage | 328 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | REGULATORY PROTEIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CYCLIN-E | - |
dc.subject.keywordPlus | COVALENT MODIFICATION | - |
dc.subject.keywordPlus | ANDROGEN RECEPTOR | - |
dc.subject.keywordPlus | DNA-REPLICATION | - |
dc.subject.keywordPlus | NUCLEAR-BODIES/ | - |
dc.subject.keywordPlus | E3 LIGASE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CONJUGATION | - |
dc.subject.keywordAuthor | human cytomegalovirus | - |
dc.subject.keywordAuthor | immediate-early protein | - |
dc.subject.keywordAuthor | protein inhibitor ofactivated STAT1 | - |
dc.subject.keywordAuthor | small ubiquitin-like modifier-1 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.