Oncostatin M이 피부섬유모세포의 증식과 기질생성에 미치는 영향Effect of Oncostatin M on Proliferation and Matrix Synthesis of Dermal Fibroblasts
- Other Titles
- Effect of Oncostatin M on Proliferation and Matrix Synthesis of Dermal Fibroblasts
- Authors
- 전경욱; 임형우; 한승규; 김우경
- Issue Date
- 2008
- Publisher
- 대한성형외과학회
- Keywords
- Oncostatin M; Dermal fibroblasts; Oncostatin M; Dermal fibroblasts
- Citation
- Archives of Plastic Surgery, v.35, no.2, pp.115 - 120
- Indexed
- KCI
- Journal Title
- Archives of Plastic Surgery
- Volume
- 35
- Number
- 2
- Start Page
- 115
- End Page
- 120
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/124616
- ISSN
- 2234-6163
- Abstract
- Purpose: Oncostatin M(OSM) is a multifunctional cytokine that belongs to the interleukin(IL)-6 family. Although there have been a number of studies that focused on the role and mechanism of OSM in various organs and tissues, there are few reports on its effect on wound healing. The final purpose of this project is to evaluate the effect of OSM on wound healing. This pilot study was designed to investigate the effect of OSM on proliferation and matrix synthesis of human dermal fibroblasts, which are the major components of the wound healing. Methods: Excess skin that was obtained from patients who underwent skin grafts, was used for this study. From this material, fibroblasts were isolated and cultured. The cultured fibroblasts were treated with one of four concentrations of OSM. The OSM concentrations used were 0, 50, 100, and 200ng/ml, respectively. After the OSM treatment, cell proliferation was determined by the MTT assay, collagen synthesis by the C1CP method, GAG levels by the Blyscan Dye method. The parameter levels of each group were compared. Results: OSM treatment increased all the components tested in the study. In particular, cell proliferation, GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). The highest increase in all the components was obtained at a 100ng/ml concentration of OSM.Conclusion: The results of the present study indicate that OSM stimulates proliferation and matrix synthesis of human dermal fibroblast and the optimal concentration for wound healing is 100ng/mL.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.