The Rat Myosin Light Chain Promoter-Driven DsRed Reporter System Allows Specific Monitoring of Bone Marrow Mesenchymal Stem Cell-Derived CardiomyocytesThe Rat Myosin Light Chain Promoter-Driven DsRed Reporter System Allows Specific Monitoring of Bone Marrow Mesenchymal Stem Cell-Derived Cardiomyocytes
- Other Titles
- The Rat Myosin Light Chain Promoter-Driven DsRed Reporter System Allows Specific Monitoring of Bone Marrow Mesenchymal Stem Cell-Derived Cardiomyocytes
- Authors
- 최승철; 임도선
- Issue Date
- 2008
- Publisher
- 사단법인 한국동물생명공학회
- Keywords
- Cardiomyogenic differentiation; DsRed; Promoter; Mesenchymal stem cells; Reporter gene; Cardiomyogenic differentiation; DsRed; Promoter; Mesenchymal stem cells; Reporter gene
- Citation
- Reproductive & Developmental biology, v.32, no.1, pp.21 - 25
- Indexed
- KCI
- Journal Title
- Reproductive & Developmental biology
- Volume
- 32
- Number
- 1
- Start Page
- 21
- End Page
- 25
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/124734
- ISSN
- 1738-2432
- Abstract
- Bone marrow mesenchymal stem cells (BMMSCs) have the capacity for self-renewal and differentiation into a variety of cell types. They represent an attractive source of cells for gene and cell therapy. The purpose of this study is to direct the specific expression of the DsRed reporter gene in Sca-1+ BMMSCs differentiated into a cardiomyogenic lineage. We constructed the prMLC-2v-DsRed vector expressing DsRed under the control of the 309 bp fragment of the rat MLC-2v 5'-flanking region. The specific expression of the DsRed reporter gene under the transcriptional control of the 309 bp fragment of the rat MLC-2v promoter was tested in 5-azacytidine treated-Sca-1+ BMMSCs over 2 weeks after the prMLC-2v-DsRed transfection. The prMLC-2v-DsRed was specifically expressed in the Sca-1+ BMMSCs with cardiomyogenic lineage differentiation, and it demonstrates that the 309 bp sequences of the rat MLC-2v 5'-flanking region is sufficient to confer cardiac specific expression on a DsRed reporter gene. The cardiac-specific promoter-driven reporter vector provides an important tool for the study of stem cell differentiation and cell replacement therapy in ischemic cardiomyopathy.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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