Optimization of PS-7 production process by Azotobacter indicus var. myxogenes L3 using the control of carbon source composition
- Authors
- Ra, C.H.; Kim, K.M.; Hoe, P.W.; Lee, S.J.; Kim, S.-K.
- Issue Date
- 2008
- Keywords
- 5-L fermentor; Azotobacter indicus var. myxogenes L3; Heteropolysaccharide-7; Whey
- Citation
- Korean Journal of Microbiology and Biotechnology, v.36, no.1, pp.61 - 66
- Indexed
- SCOPUS
KCI
- Journal Title
- Korean Journal of Microbiology and Biotechnology
- Volume
- 36
- Number
- 1
- Start Page
- 61
- End Page
- 66
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/125323
- ISSN
- 1598-642X
- Abstract
- The proteins in whey are separated and used as food additives. The remains (mainly lactose) are spray-dried to produce sweet whey powder, which is widely used as an additive for animal feed. Sweet whey powder is also used as a carbon source for the production of valuable products such as polysaccharides. Glucose, fructose, galactose, and sucrose as asupplemental carbon source were evaluated for the production of PS-7 from Azotobacter indicus var.myxogenes L3 grown on whey based MSM media. Productions of PS-7 with 2% (w/v) fructose and sucrose were 2.05 and 2.31 g/L, respectively. The highest production of PS-7 was 2.82 g/L when 2% (w/v) glucose was used as the carbon source. Galactose showed low production of PS-7 among the carbon sources tested. The effects of various carbon sources addition to whey based MSM medium showed that glucose could be the best candidate for the enhancement of PS-7 production using whey based MSM medium. To evaluate the effect of glucose addition to whey based media on PS-7 production, fermentations with whey and glucose mixture (whey 1, 2, 3%; whey 1% + glucose 1%, whey 1% + glucose 2% and glucose 2%, w/v) were carried out. Significant enhancement of PS-7 production with addition of 1% (w/v) and 2% (w/v) glucose in 1% (w/v) whey media was observed. The PS-7 concentration of 2% glucose added whey lactose based medium was higher than that of 1% glucose addition, however, the product yield YP/S was higher in 1% glucose added whey lactose based MSM medium. Therefore, the optimal condition for the PS-7 production from the Azotobacter indicus var.myxogenes L3, was 1% glucose addition to 1% whey lactose MSM medium.
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