Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits
- Authors
- Vukoti, Krishna Moorthy; Lee, Won-Kyu; Kim, Ho-Jun; Kim, Ick Young; Yang, Eun Gyeong; Lee, Cheolju; Yu, Yeon Gyu
- Issue Date
- 20-6월-2007
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- leukotriene B4; hBLT2; G-protein; intracellular loop; GPCR-G protein interaction
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.28, no.6, pp.1005 - 1009
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 28
- Number
- 6
- Start Page
- 1005
- End Page
- 1009
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/125758
- ISSN
- 0253-2964
- Abstract
- Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G alpha protein including G alpha i1, G alpha i2, G alpha i3, G alpha s1, G alpha o1, and G alpha z. Among the tested G alpha subunits, G alpha i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (K-D) of 5.0 x 10(-6) M. These results suggest that G alpha i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G alpha i3.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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