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Rapid DNA hybridization analysis using a PDMS microfluidic sensor and a molecular beacon

Authors
Kim, SungyongChen, LingxinLee, SangyeopSeong, Gi HunChoo, JaebumLee, Eun KyuOh, Chil-HwanLee, Sanghoon
Issue Date
4월-2007
Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
Citation
ANALYTICAL SCIENCES, v.23, no.4, pp.401 - 405
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL SCIENCES
Volume
23
Number
4
Start Page
401
End Page
405
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125788
DOI
10.2116/analsci.23.401
ISSN
0910-6340
Abstract
A rapid DNA analysis has been developed based on a fluorescence intensity change of a molecular beacon in a PDMS microfluidic channel. Recently, we reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). However, there are some limitations in its application to real DNA samples because the target DNA must be labelled with a suitable fluorescent dye. To resolve this problem, we have developed a new DNA microfluidic sensor using a molecular beacon. By monitoring the change in the restored fluorescence intensity along the channel length, it is possible to rapidly detect any hybridization of the molecular beacon to the target DNA. In this case, the target DNA does not need to be labelled. Our experimental results demonstrate that this microfluidic sensor using a molecular beacon is a promising diagnostic tool for rapid DNA hybridization analysis.
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College of Medicine > Department of Medical Science > 1. Journal Articles
College of Health Sciences > School of Biomedical Engineering > 1. Journal Articles

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