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Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells

Authors
Kim, JungjuKim, In SookCho, Tae HyungLee, Kyu BackHwang, Soon JungTae, GiyoongNoh, InsupLee, Sang HoonPark, YongdooSun, Kyung
Issue Date
4월-2007
Publisher
ELSEVIER SCI LTD
Keywords
hyaluronic acid; hydrogel; bone regeneration; bone morphogenic protein; mesenchymal stem cells
Citation
BIOMATERIALS, v.28, no.10, pp.1830 - 1837
Indexed
SCIE
SCOPUS
Journal Title
BIOMATERIALS
Volume
28
Number
10
Start Page
1830
End Page
1837
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125789
DOI
10.1016/j.biomaterials.2006.11.050
ISSN
0142-9612
Abstract
Acrylated hyaluronic acid (HA) was used as a scaffold for bone morphogenic protein-2 (BMP-2) and human mesenchymal stem cells (hMSCs) for rat calvarial defect regeneration. HA was acrylated by two-step reactions: (1) introduction of an amine group using adipic acid dihydrazide (ADH); (2) acrylation by N-acryloxysuccinimide. Tetrathiolated poly(ethylene) glycol (PEG-SH4) was used as a crosslinker by a Michael-type addition reaction and the hydrogel was formed within 10 min under physiological conditions. This hydrogel is degraded completely by 100 U/ml hyaluronidase in vitro. hMSCs and/or BMP-2 was added during gelation. Cellular viability in vitro was increased up to 55% in the hydrogels with BMP-2 compared with the control. For in vivo calvarial defect regeneration, five different samples (i.e., control, hydrogel, hydrogel with BMP-2, hydrogel with MSCs, and hydrogel with BMP-2 and MSCs) were implanted for 4 weeks. The histological results demonstrated that the hydrogels with BMP-2 and MSCs had the highest expression of osteocalcin and mature bone formation with vascular markers, such as CD31 and vascular endothelial growth factors, compared with the other samples. This study demonstrated that HA base hydrogel can be used for cell and growth factor carriers for tissue regeneration. (c) 2006 Elsevier Ltd. All rights reserved.
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