Fast and sensitive DNA analysis using changes in the FRET signals of molecular beacons in a PDMS microfluidic channel
DC Field | Value | Language |
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dc.contributor.author | Jung, Jaehyun | - |
dc.contributor.author | Chen, Lingxin | - |
dc.contributor.author | Lee, Sangyup | - |
dc.contributor.author | Kim, Sungyong | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.contributor.author | Choo, Jaebum | - |
dc.contributor.author | Lee, Eun Kyu | - |
dc.contributor.author | Oh, Chil-Hwan | - |
dc.contributor.author | Lee, Sanghoon | - |
dc.date.accessioned | 2021-09-09T17:22:45Z | - |
dc.date.available | 2021-09-09T17:22:45Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/125791 | - |
dc.description.abstract | A new DNA hybridization analytical method using a microfluidic channel and a molecular beacon-based probe (MB-probe) is described. A stem-loop DNA oligonucleotide labeled with two fluorophores at the 5' and 3' termini (a donor dye, TET, and an acceptor dye, TAMRA, respectively) was used to carry out a fast and sensitive DNA analysis. The MB-probe utilized the specificity and selectivity of the DNA hairpin-type probe DNA to detect a specific target DNA of interest. The quenching of the fluorescence resonance energy transfer (FRET) signal between the two fluorophores, caused by the sequence-specific hybridization of the MB-probe and the target DNA, was used to detect a DNA hybridization reaction in a poly(dimethylsiloxane) (PDMS) microfluidic channel. The azoospermia gene, DYS 209, was used as the target DNA to demonstrate the applicability of the method. A simple syringe pumping system was used for quick and accurate analysis. The laminar flow along the channel could be easily controlled by the 3-D channel structure and flow speed. By injecting the MB-probe and target DNA solutions into a zigzag-shaped PDMS microfluidic channel, it was possible to detect their sequence-specific hybridization. Surface-enhanced Raman spectroscopy (SERS) was also used to provide complementary evidence of the DNA hybridization. Our data show that this technique is a promising real-time detection method for label-free DNA targets in the solution phase. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | SURFACE-ENHANCED RAMAN | - |
dc.subject | TOTAL ANALYSIS SYSTEMS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | SCATTERING | - |
dc.subject | SILVER | - |
dc.subject | NANOPARTICLES | - |
dc.subject | CHIP | - |
dc.title | Fast and sensitive DNA analysis using changes in the FRET signals of molecular beacons in a PDMS microfluidic channel | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Chil-Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Sanghoon | - |
dc.identifier.doi | 10.1007/s00216-007-1158-6 | - |
dc.identifier.scopusid | 2-s2.0-33947610168 | - |
dc.identifier.wosid | 000245123600002 | - |
dc.identifier.bibliographicCitation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.387, no.8, pp.2609 - 2615 | - |
dc.relation.isPartOf | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.volume | 387 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2609 | - |
dc.citation.endPage | 2615 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | SURFACE-ENHANCED RAMAN | - |
dc.subject.keywordPlus | TOTAL ANALYSIS SYSTEMS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | SCATTERING | - |
dc.subject.keywordPlus | SILVER | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordAuthor | fluorescence resonance energy transfer | - |
dc.subject.keywordAuthor | molecular beacon | - |
dc.subject.keywordAuthor | lab-on-a-chip | - |
dc.subject.keywordAuthor | surface-enhanced Raman | - |
dc.subject.keywordAuthor | DNA hybridization | - |
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