Noviherbaspirillum pedocola sp. nov., isolated from oil-contaminated experimental soil

Abstract

An orange-coloured, rod-shaped, and aerobic bacterial strain DKR-6 (T) was isolated from oil-contaminated experimental soil. The strain was Gram-stain-negative, catalase and oxidase positive, and grew at temperature 10-42 degrees C, at pH 5.5-9.5, and at 0-3.0% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence analysis suggested that the strain DKR-6 (T) was affiliated to the genus Noviherbaspirillum, with the closest species being Noviherbaspirillum massiliense JC206(T) (96.3% sequence similarity). The chemotaxonomic profiles revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine as the principal polar lipids; C-16:0, C-17:0 cyclo, summed feature 3 (C-16:1 omega 7c and/or C-16: 1 omega 6c), and summed feature 8 (C-18:1 omega 7c/or C-18:1 omega 6c) as the main fatty acids; and Q-8 as a sole ubiquinone. The DNA G + C content was 61.6%. The polyphasic taxonomic features illustrated in this study clearly implied that strain DKR-6 (T) represents a novel species in the genus Noviherbaspirillum, for which the name Noviherbaspirillum pedocola sp. nov. is proposed with the type strain DKR-6 (T) (= KACC 22074 (T) = NBRC 114727 (T)).